Dehydration

Question 1

Removal of intracellular and extracellular water from the fixed tissue in preparation for impregnation procedure

Answer 1

Dehydration

Question 2

Widely used dehydrating agent in the lab

Answer 2

Alcohol

Question 3

Recommended ration of dehydrating agent to tissue

Answer 3

10:1

Question 4

Done after fixation and prior to wax impregnation

Answer 4

Dehydration

Question 5

Characteristics of an ideal dehydrating ageng

Answer 5

1. Dehydrate fast
2. NOT Evaporate fast
3. Evaporate fatty tissues
4. NOT harden the tissue excessively
5. NOT remove stains
6. NOT toxic to the body
7. NOT a fire hazard

Question 6

Use for routine dehydration

Answer 6

Ethyl alcohol (ethanol)

Question 7

Clear, colorless and flammable

Answer 7

Ethyl alcohol (ethanol)

Question 8

Best dehydrating agent

Answer 8

Ethyl alcohol (ethanol)

Question 9

Ethanol is fast acting because is mixes with water and many organic solvent and penetrates the tissue easily (true or false)

Answer 9

True

Question 10

Not poisonous and not very expensive

Answer 10

Ethyl alcohol (ethanol)

Question 11

Substitute for ethanol

Answer 11

Isopropyl alcohol

Question 12

Dehydrating agent for blood and tissue films

Answer 12

Methyl alcohol

Question 13

Methyl alcohol is a toxic dehydrating agent (true or false)

Answer 13

True

Question 14

Fixative for smear preparations (bone marrow)

Answer 14

Methyl alcohol

Question 15

Utilize in plants and animal micro-techniques

Answer 15

Butyl alcohol

Question 16

A slow dehydrating agent and is recommended for non-urgent works and it also produces less shrinkage and hardening

Answer 16

Butyl alcohol

Question 17

The strength of the initial alcohol required will depend upon the size, nature of the tissue and the fixative used. (True or false)

Answer 17

True

Question 18

Recommended starting concentration of alcohol in delicate tissues

Answer 18

30%

Question 19

Optimum temperature for storing tissue samples

Answer 19

70-80%

Question 20

Clear, colorless fluid that mixes with water, ethanol, and most organic solvents

Answer 20

Acetone

Question 21

More miscible with epoxy resins than alcohol

Answer 21

Acetone

Question 22

Cheap and rapid-acting but has poor penetration

Answer 22

Acetone

Question 23

Used for urgent biopsies and could remove lipids from the tissue

Answer 23

Acetone

Question 24

Dehydrating time of acetone

Answer 24

30mins to 2hrs

Question 25

Dioxane is also known as?

Answer 25

Diethylene dioxide

Question 26

Readily miscible with water, melted paraffin, alcohol and xylol

Answer 26

Dioxane

Question 27

Tissues can be left for long periods of time and tends to ribbon poorly

Answer 27

Dioxane

Question 28

Expensive and extremely dangerous

Answer 28

Dioxane

Question 29

Dioxane should be used readily and the room should be not properly ventilated because it produces vapors

Answer 29

False (not be used readily and the room should be properly ventilated)

Question 30

Cellosolve is also known as?

Answer 30

Ethylene glycol monoethyl ether

Question 31

Tissue samples with cellosolve can be stored for months without producing hardening/distortion unlike alcohol (true or false)

Answer 31

True

Question 32

Cellosolve is combustible at how many degree Farenheit

Answer 32

110-120degree F (43.3-48.8degree C)

Question 33

Toxic by inhalation, skin contact, and targets reproductive, fetal, urinary and blood systems

Answer 33

Cellosolve

Question 34

This can be used in cellosolve instead of Ethylene glycol monoethyl ether to avoid side effects

Answer 34

Propylene-based glycol ether

Question 35

Removes water very readily

Answer 35

Triethyl phosphate

Question 36

Produces very little distortion and hardening

Answer 36

Triethyl phosphate

Question 37

The amount of dehydrating agent should not be less than 10 times the volume of the tissue (true or false)

Answer 37

True

Question 38

Soluble in alcohol, water, ether, benzene, chloroform, acetone and xylene

Answer 38

Triethyl phosphate

Question 39

Both dehydrating and alcohol agent like dioxane

Answer 39

Tetrahydrofuran

Question 40

Could dissolve fat and may be used for refixing, clearing, and dehydrating paraffin section before and after staining

Answer 40

Tetrahydrofuran

Question 41

Miscible with lower alcohols, ether, chloroform, acetone, benzene, and xylene

Answer 41

Tetrahydrofuran

Question 42

It will not dissolve aniline dyes and is toxic when ingested or inhaled

Answer 42

Tetahydrofuran

Question 43

An eye and skin irritant

Answer 43

Tetrahydrofuran

Question 44

Prolonged exposure of THD up to 6months may cause conjunctival irritation (true or false)

Answer 44

True

Question 45

Act as a tissue softener for hard tissue

Answer 45

4% phenol

Question 46

Added to each of the 95% ethanol baths

Answer 46

4% phenol