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HISTOPATH LEC > Dehydration > Flashcards

Dehydration Flashcards

1
Q

Removal of intracellular and extracellular water from the fixed tissue in preparation for impregnation procedure

A

Dehydration

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2
Q

Widely used dehydrating agent in the lab

A

Alcohol

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3
Q

Recommended ration of dehydrating agent to tissue

A

10:1

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4
Q

Done after fixation and prior to wax impregnation

A

Dehydration

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5
Q

Characteristics of an ideal dehydrating ageng

A
  1. Dehydrate fast
  2. NOT Evaporate fast
  3. Evaporate fatty tissues
  4. NOT harden the tissue excessively
  5. NOT remove stains
  6. NOT toxic to the body
  7. NOT a fire hazard
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6
Q

Use for routine dehydration

A

Ethyl alcohol (ethanol)

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7
Q

Clear, colorless and flammable

A

Ethyl alcohol (ethanol)

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8
Q

Best dehydrating agent

A

Ethyl alcohol (ethanol)

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9
Q

Ethanol is fast acting because is mixes with water and many organic solvent and penetrates the tissue easily (true or false)

A

True

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10
Q

Not poisonous and not very expensive

A

Ethyl alcohol (ethanol)

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11
Q

Substitute for ethanol

A

Isopropyl alcohol

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12
Q

Dehydrating agent for blood and tissue films

A

Methyl alcohol

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13
Q

Methyl alcohol is a toxic dehydrating agent (true or false)

A

True

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14
Q

Fixative for smear preparations (bone marrow)

A

Methyl alcohol

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15
Q

Utilize in plants and animal micro-techniques

A

Butyl alcohol

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16
Q

A slow dehydrating agent and is recommended for non-urgent works and it also produces less shrinkage and hardening

A

Butyl alcohol

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17
Q

The strength of the initial alcohol required will depend upon the size, nature of the tissue and the fixative used. (True or false)

A

True

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18
Q

Recommended starting concentration of alcohol in delicate tissues

A

30%

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19
Q

Optimum temperature for storing tissue samples

A

70-80%

20
Q

Clear, colorless fluid that mixes with water, ethanol, and most organic solvents

A

Acetone

21
Q

More miscible with epoxy resins than alcohol

A

Acetone

22
Q

Cheap and rapid-acting but has poor penetration

A

Acetone

23
Q

Used for urgent biopsies and could remove lipids from the tissue

A

Acetone

24
Q

Dehydrating time of acetone

A

30mins to 2hrs

25
Q

Dioxane is also known as?

A

Diethylene dioxide

26
Q

Readily miscible with water, melted paraffin, alcohol and xylol

A

Dioxane

27
Q

Tissues can be left for long periods of time and tends to ribbon poorly

A

Dioxane

28
Q

Expensive and extremely dangerous

A

Dioxane

29
Q

Dioxane should be used readily and the room should be not properly ventilated because it produces vapors

A

False (not be used readily and the room should be properly ventilated)

30
Q

Cellosolve is also known as?

A

Ethylene glycol monoethyl ether

31
Q

Tissue samples with cellosolve can be stored for months without producing hardening/distortion unlike alcohol (true or false)

A

True

32
Q

Cellosolve is combustible at how many degree Farenheit

A

110-120degree F (43.3-48.8degree C)

33
Q

Toxic by inhalation, skin contact, and targets reproductive, fetal, urinary and blood systems

A

Cellosolve

34
Q

This can be used in cellosolve instead of Ethylene glycol monoethyl ether to avoid side effects

A

Propylene-based glycol ether

35
Q

Removes water very readily

A

Triethyl phosphate

36
Q

Produces very little distortion and hardening

A

Triethyl phosphate

37
Q

The amount of dehydrating agent should not be less than 10 times the volume of the tissue (true or false)

A

True

38
Q

Soluble in alcohol, water, ether, benzene, chloroform, acetone and xylene

A

Triethyl phosphate

39
Q

Both dehydrating and alcohol agent like dioxane

A

Tetrahydrofuran

40
Q

Could dissolve fat and may be used for refixing, clearing, and dehydrating paraffin section before and after staining

A

Tetrahydrofuran

41
Q

Miscible with lower alcohols, ether, chloroform, acetone, benzene, and xylene

A

Tetrahydrofuran

42
Q

It will not dissolve aniline dyes and is toxic when ingested or inhaled

A

Tetahydrofuran

43
Q

An eye and skin irritant

A

Tetrahydrofuran

44
Q

Prolonged exposure of THD up to 6months may cause conjunctival irritation (true or false)

A

True

45
Q

Act as a tissue softener for hard tissue

A

4% phenol

46
Q

Added to each of the 95% ethanol baths

A

4% phenol

HISTOPATH LEC (4 decks)

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