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HISTOPATH LEC > 2nd LLE > Flashcards

2nd LLE Flashcards

1
Q

Used for delicate specimens and frozen tissue sections because it prevents fragmentation of tough and friable tissues

A

Gelatin impregnation

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2
Q

Embedding mold recommended for busy routine laboratories

A

Plastic ice trays

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3
Q

Method of paraffin wax impregnation gives the fastest result

A

Vacuum processing

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4
Q

Melting point of wax commonly used for routine procedures

A

56 C

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5
Q

Process by which tissue is arranged in precise position in the mold before embedding

A

Orientation

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6
Q

A series of interlocking plates resting on a flat metal base

A

Compound embedding unit

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7
Q

Gives superior results for a light microscopic studies in hard tissues like undecalcified bones

A

Plastic embedding

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8
Q

Acrylic plastic is for?

A

Light microscopy

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9
Q

Polyesters plastic is for?

A

Electron microscopy

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10
Q

At what temperature should a paraffin oven be maintained when used for impregnation

A

2-5 centrigade above melting point

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11
Q

Melting point of highly purified paraffin and synthetic plastic polymers

A

56-57 C

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12
Q

Most rapid clearing agent suitable for urgent biopsies

A

Xylol

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13
Q

It causes the minimum shrinkage of tissues but dissolves aniline dyes and celloidin

A

Clove oil

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14
Q

Recommended for embryos and very delicate specimen

A

Cedarwood oil

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15
Q

Considered carcinogenic and may damage the bone marrow resulting in a plastic anemia

A

Benzene

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16
Q

Carbon tetrachloride has properties similar to?

A

Chloroform

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17
Q

Recommended for fibroid and decalcified tissues

A

Chloroform

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18
Q

Characteristics of chloroform makes it unsuitable as a clearing agent

A

It does not make the tissue transparent

19
Q

What clearing agent should be used when double embedding techniques are required

A

Methyl benzoate and Methyl salicylate

20
Q

If both xylene and benzene are not available, which clearing agent can be used as a substitute?

A

Toluene

21
Q

Refractive index of a plastic slide

A

1.518

22
Q

Dehydrating agent that is toxic and is formally employed for blood and tissue films and smear preparation

A

Methyl alcohol

23
Q

An excellent dehydrating and clearing agent that is readily miscible in water, melted paraffin, alcohol and xylol but is expensive and extremely dangerous

A

Dioxane

24
Q

Process of removing intracellular and extracellular water from the tissue following fixation and prior to impregnation

A

Dehydration

25
Q

What is added to the dehydrating agent that will act as a softener for hard tissues such as tendon, nail, or dense fibrous tissue

A

4% phenol

26
Q

What dehydrating agent is combustible at 110-120 F and toxic by inhalation, skin contact, and ingestion

A

Ethylene glycol ethers

27
Q

How does dehydration start with the concentration of the dehydrating agent

A

Increasing concentration

28
Q

Recommended dehydrating agent for routine purpose

A

Alcohol

29
Q

Dehydrating agent dehydrated rapidly to the point where tissue may be transferred from water and NSS directly to the dehydrating agent and stored there for months without hardening/distorting

A

Cellosolve and Ethylene glycol monoethyl ether

30
Q

Recommended for cutting extremely hard and rough tissue block

A

Sliding microtome

31
Q

Type of microtome reduces tissue sections of 0.5 inches in thickness

A

Ultrathin microtome

32
Q

How many double strokes are presently given to each side of the knife in a mechanical knife sharpener

A

30 strokes

33
Q

Used for cutting or trimming and/or for semi-thin sectioning of tissue blocks for electron microscopy

A

Glass knives

34
Q

Process of removing the burr form during honing

A

Stropping

35
Q

What should be the clearance angle made to prevent uneven tissue sections

A

5-10 degree

36
Q

Only used for badly nicked knives

A

Fine carborundum

37
Q

What microtome can be mounted on a bench-top

A

Freezing microtome

38
Q

How many double strokes are required in the stropping process

A

40-120

39
Q

First and most critical step in histotechnology which involves preserving the fresh tissue examination

A

Fixation

40
Q

The step that gives the tissue a translucent appearance

A

Clearing

41
Q

According to United States of CDC, regular hand washing helps eliminate the spreading of germs (true or false)

A

True

42
Q

Prolonged fixation makes tissue blocks brittle and hard (true or false)

A

True

43
Q

The medium used in embedding is the same medium used to infiltrate the tissue samples (true or false)

A

True

44
Q

Cutting away the excess wax from the sides, top, and bottom of the tissue block until perfectly leveled on all sides of parallel to each other almost to the edge of the tissue

A

Coarse trimming

HISTOPATH LEC (4 decks)

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