2nd LLE

Question 1

Used for delicate specimens and frozen tissue sections because it prevents fragmentation of tough and friable tissues

Answer 1

Gelatin impregnation

Question 2

Embedding mold recommended for busy routine laboratories

Answer 2

Plastic ice trays

Question 3

Method of paraffin wax impregnation gives the fastest result

Answer 3

Vacuum processing

Question 4

Melting point of wax commonly used for routine procedures

Answer 4

56 C

Question 5

Process by which tissue is arranged in precise position in the mold before embedding

Answer 5

Orientation

Question 6

A series of interlocking plates resting on a flat metal base

Answer 6

Compound embedding unit

Question 7

Gives superior results for a light microscopic studies in hard tissues like undecalcified bones

Answer 7

Plastic embedding

Question 8

Acrylic plastic is for?

Answer 8

Light microscopy

Question 9

Polyesters plastic is for?

Answer 9

Electron microscopy

Question 10

At what temperature should a paraffin oven be maintained when used for impregnation

Answer 10

2-5 centrigade above melting point

Question 11

Melting point of highly purified paraffin and synthetic plastic polymers

Answer 11

56-57 C

Question 12

Most rapid clearing agent suitable for urgent biopsies

Answer 12

Xylol

Question 13

It causes the minimum shrinkage of tissues but dissolves aniline dyes and celloidin

Answer 13

Clove oil

Question 14

Recommended for embryos and very delicate specimen

Answer 14

Cedarwood oil

Question 15

Considered carcinogenic and may damage the bone marrow resulting in a plastic anemia

Answer 15

Benzene

Question 16

Carbon tetrachloride has properties similar to?

Answer 16

Chloroform

Question 17

Recommended for fibroid and decalcified tissues

Answer 17

Chloroform

Question 18

Characteristics of chloroform makes it unsuitable as a clearing agent

Answer 18

It does not make the tissue transparent

Question 19

What clearing agent should be used when double embedding techniques are required

Answer 19

Methyl benzoate and Methyl salicylate

Question 20

If both xylene and benzene are not available, which clearing agent can be used as a substitute?

Answer 20

Toluene

Question 21

Refractive index of a plastic slide

Answer 21

1.518

Question 22

Dehydrating agent that is toxic and is formally employed for blood and tissue films and smear preparation

Answer 22

Methyl alcohol

Question 23

An excellent dehydrating and clearing agent that is readily miscible in water, melted paraffin, alcohol and xylol but is expensive and extremely dangerous

Answer 23

Dioxane

Question 24

Process of removing intracellular and extracellular water from the tissue following fixation and prior to impregnation

Answer 24

Dehydration

Question 25

What is added to the dehydrating agent that will act as a softener for hard tissues such as tendon, nail, or dense fibrous tissue

Answer 25

4% phenol

Question 26

What dehydrating agent is combustible at 110-120 F and toxic by inhalation, skin contact, and ingestion

Answer 26

Ethylene glycol ethers

Question 27

How does dehydration start with the concentration of the dehydrating agent

Answer 27

Increasing concentration

Question 28

Recommended dehydrating agent for routine purpose

Answer 28

Alcohol

Question 29

Dehydrating agent dehydrated rapidly to the point where tissue may be transferred from water and NSS directly to the dehydrating agent and stored there for months without hardening/distorting

Answer 29

Cellosolve and Ethylene glycol monoethyl ether

Question 30

Recommended for cutting extremely hard and rough tissue block

Answer 30

Sliding microtome

Question 31

Type of microtome reduces tissue sections of 0.5 inches in thickness

Answer 31

Ultrathin microtome

Question 32

How many double strokes are presently given to each side of the knife in a mechanical knife sharpener

Answer 32

30 strokes

Question 33

Used for cutting or trimming and/or for semi-thin sectioning of tissue blocks for electron microscopy

Answer 33

Glass knives

Question 34

Process of removing the burr form during honing

Answer 34

Stropping

Question 35

What should be the clearance angle made to prevent uneven tissue sections

Answer 35

5-10 degree

Question 36

Only used for badly nicked knives

Answer 36

Fine carborundum

Question 37

What microtome can be mounted on a bench-top

Answer 37

Freezing microtome

Question 38

How many double strokes are required in the stropping process

Answer 38

40-120

Question 39

First and most critical step in histotechnology which involves preserving the fresh tissue examination

Answer 39

Fixation

Question 40

The step that gives the tissue a translucent appearance

Answer 40

Clearing

Question 41

According to United States of CDC, regular hand washing helps eliminate the spreading of germs (true or false)

Answer 41

True

Question 42

Prolonged fixation makes tissue blocks brittle and hard (true or false)

Answer 42

True

Question 43

The medium used in embedding is the same medium used to infiltrate the tissue samples (true or false)

Answer 43

True

Question 44

Cutting away the excess wax from the sides, top, and bottom of the tissue block until perfectly leveled on all sides of parallel to each other almost to the edge of the tissue

Answer 44

Coarse trimming