Adhesive

Question 1

Process of sealing the margins of the coverslip to prevent the escape of fluid or semi-fluid mounts and evaporation of mountant, to immobilize the coverslip and to prevent sticking of the slides upon storage

Answer 1

Ringing

Question 2

2 parts paraffin wax + 4-9 parts of powdered colophonium resin

Answer 2

Kronig cement

Question 3

10 parts paraffin wax + 40 parts colophonium resin

Answer 3

Du noyer’s wax

Question 4

Used in preps that have been dehydrated and cleared in xylene and toluene

Answer 4

Resinous mounting media

Question 5

Recommended for majority of staining method

Answer 5

Resinous mounting media

Question 6

Refractive index of Canada Balsam

Answer 6

1.524

Question 7

Made neutral or acidic + excess amount of Calcium carbonate or salicylic acid

Answer 7

Canada Balsam

Question 8

Recommended for whole mounts and for thick sections because it does not shrink much

Answer 8

Canada Balsam

Question 9

At what degree of Incubation will the natural resin from the Canadian tree dissolve in Xylene?

Answer 9

37 C or 58C in paraffin oven

Question 10

Refractive index of DPX

Answer 10

1.532

Question 11

For small tissue sections only

Answer 11

DPX

Question 12

Mounting media that preserves stains well

Answer 12

XAM

Question 13

Refractive index of XAM

Answer 13

1.52

Question 14

Resinous Mounting Media that is most commonly used, solidify within 20 mins

Answer 14

EUKITT

Question 15

Made up of Gelatin, Glyserin jelly or gum Arabic

Answer 15

Aqueous mounting media

Question 16

To prevent cracking and drying of prep

Answer 16

Glycerol

Question 17

Used to increase refractive index

Answer 17

Sugar

Question 18

Refractive index of water

Answer 18

1. 333

Question 19

Refractive index of Glycerin

Answer 19

1.46

Question 20

Aqueous mounting media for preservative action, sets hard, will keep sections mounted for years

Answer 20

Glycerin

Question 21

Standard mounting medium used when dehydration and clearing with xylene cannot be made

Answer 21

Glycerin jelly

Question 22

Prevents metachromatic stain to diffuse from the section

Answer 22

Fructose syrup

Question 23

Is readily available from outdated blood source stored in blood banks, dispensed into sterile tubes of 0.5 ml each

Answer 23

Plasma

Question 24

Added to slide before the application of coverslip for protection

Answer 24

Mounting medium

Question 25

The ratio of velocity of light in a vacuum to its velocity in a specified medium

Answer 25

Refractive index

Question 26

An aqueous detergent which can be purchased as a 0.1% solution

Answer 26

Poly-l-lysine

Question 27

Ratio of Poly-L-Lysine

Answer 27

1:10

Question 28

Best adhesive and better than Poly-L-Lysine

Answer 28

Apes

Question 29

Useful for cytology especially for cystospin preps of proteinacious or bloody material

Answer 29

Apes

Question 30

Most commonly used because it is easy to make, convenient and inexpensive

Answer 30

Mayer’s Egg Albumin

Question 31

Essential for methods that require exposure of sections to acids and alkalis during staining

Answer 31

Adhesive

Question 32

Ration of Mayer’s Egg Albumin

Answer 32

1:1

Question 33

Mayer’s Egg Albumin is made up of?

Answer 33

50ml egg white, 50ml glycerol, 100mg crystals of thymol

Question 34

Dried albumin is made up of?

Answer 34

5g dried albumin to 5g sodium chloride

Question 35

Best alternative adhesive

Answer 35

1% Gelatin

Question 36

Not necessary for routine staining, provided that the slides are clean and free from grease

Answer 36

Adhesive

Question 37

Gelatin-Formaldehyde mixture

Answer 37

5ml 1% Gelatin, 5ml 2% formaldehyde

Question 38

Good only for temporary mounting

Answer 38

Water

Question 39

Preferred over DPX and is soluble in xylene and is used as a 60% solution

Answer 39

Clarite/Clarite X

Question 40

Refractive index of Clarite/Clarite X

Answer 40

1.544