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Microbiology Lab > Culturing terminology and streak plating quiz > Flashcards

Culturing terminology and streak plating quiz Flashcards

1
Q

what is a culture?

A

the growth of bacteria in a lab setting

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2
Q

what is inoculum?

A

placing bacteria in a growth medium

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3
Q

what is a growth medium (media)?

A

supplies nutrients and energy requirements for microbial growth

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4
Q

what are examples of the types of nutrients growth medium would contain?

A

carbon source - glucose
nitrogen
vitamins
water

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5
Q

what are the two forms media come in?

A

solid
liquid

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6
Q

what is another name for solid media?

A

agar

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7
Q

what is another name for liquid media?

A

broth

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8
Q

what are the three forms agar can be displayed?

A

angled in a test tube (slant)
flat top in a test tube (stab)
in a petri dish (plate)

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9
Q

what is the standard solid media for growth?

A

Tryptic soy agar (TSA)

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10
Q

what does tryptic soy agar contain?

A

tryptones -(amino acids)
soy contains: glucose, nitrogen and salts

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11
Q

How can liquid media be displayed?

A

can be clear -> indicating no growth
can be cloudy (turbid) -> indicates there is growth

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12
Q

what is the standard liquid media for growth?

A

tryptic soy broth (TSB)

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13
Q

what is the goal of streak plating?

A

to isolate one type of microbe

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14
Q

what were the bacteria we used in our streak?

A

Staphylococcus aureus
Escherichia coli

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15
Q

what was the first step of streak plating?

A

take one loopful of bacteria and spread over 1/4 of plate

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16
Q

what do we do between each main step of streak plating? Why?

A

flame the loop
to reduce the number of microbes as we progress each step

17
Q

what was the second main step of streak plating?

A

after flaming loop, start in zone 1 and spread bacteria over another 1/4 of plate

18
Q

what was the third main step of streak plating?

A

after flaming loop, start in zone 2 and spread bacteria over another 1/4 of plate

19
Q

what was the fourth main step of streak plating?

A

after flaming loop, start in zone 3 and spread bacteria last 1/4 of plate, leaving a tail in between (but not touching) all the other zones

Microbiology Lab (11 decks)

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