Gram + Project Wednesday Quiz Flashcards
What does the Blood agar plate test for?
For hemolysin production
What color is the blood agar plate before inoculating?
Red
What does the blood agar plate contain to give it its color?
5% sheep’s blood
What tools did we use to inoculate the blood agar plate? what technique?
Cotton swab
Zag plating technique
What is the indication of a positive result for the blood agar plate?
What does this indicate?
What is the pattern of growth called?
Red color disappears under colonies
Indicates that hemolysin production occurred
Beta pattern = clearance zones
What is the indication of a negative result for the blood agar plate?
What does this indicate?
What is the pattern of growth called?
No color change (growth remains red)
Indicates no hemolysin was produced
Gamma pattern = no clearance zone
What does the trehalose stab test for?
For trehalose fermentation
What is trehalose?
Odd plant carbohydrate
What tools did we use in the trehalose experiment?
What was the type of solid media?
Needle
stab
What was the color of the trehalose stab?
What gave it this color?
Red
Phenol red which is a pH indicator
What gave a pos/neg result for the trehalose stab?
What did this mean for the pH?
Pos: yellow color change = decrease in pH
Neg: color remains red = neutral
What did the sodium chloride broth test for?
Growth in high salinity media
What was the percent of sodium chloride in the broth?
6.5 %
What was the color of the sodium chloride broth?
What gave it this color?
Purple
Bromocresol purple = pH indicator
What tools did we use for the sodium chloride broth?
Loop
