Gram + Project Monday Quiz Flashcards
what does the novobiocin test for?
tests for resistance to novobiocin (antibiotic)
what is novobiocin? how does it work?
an antibiotic that is a DNA synthesis inhibitor
what is a pos/neg for the novobiocin test?
pos: no death zone = resistant
neg: larger death zone > 4mm = susceptible
what plate did we use for the novobiocin test? what tools/technique did we use?
plain TSA inoculated with our bacteria/ novobiocin paper
- cotton swab
- lawn plate
what did the gelatin stab test for? what does this mean if tested positive?
for gelatin protein utilization
- protease enzyme will break down the gelatin protein into its amino acids
what is the pos/neg results for the gelatin stab?
pos: liquid after chilled = break down of gelatin protein occurred
neg: remained solid after chilled = break down did not occur
what tool did we use for the gelatin stab?
needle
what did the nitrate broth test for?
tests for nitrate respiration
nitrate is an oxidized salt, able to accept electrons
what is the tool we used for the nitrate test?
loop
what is the equation for nitrate being successfully oxidized in the nitrate test?
what did the nitrate test look like before inoculating? what else was inside tube? why?
clear
Durham tube inside
to catch gas if formed
what is the pos/neg result for the nitrate broth test?
pos: color change to red and possibly gas bubbles formed inside Durham tube
Neg: color remains clear and no bubbles
