Adhesive Flashcards

1
Q

Process of sealing the margins of the coverslip to prevent the escape of fluid or semi-fluid mounts and evaporation of mountant, to immobilize the coverslip and to prevent sticking of the slides upon storage

A

Ringing

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2
Q

2 parts paraffin wax + 4-9 parts of powdered colophonium resin

A

Kronig cement

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3
Q

10 parts paraffin wax + 40 parts colophonium resin

A

Du noyer’s wax

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4
Q

Used in preps that have been dehydrated and cleared in xylene and toluene

A

Resinous mounting media

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5
Q

Recommended for majority of staining method

A

Resinous mounting media

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6
Q

Refractive index of Canada Balsam

A

1.524

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7
Q

Made neutral or acidic + excess amount of Calcium carbonate or salicylic acid

A

Canada Balsam

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8
Q

Recommended for whole mounts and for thick sections because it does not shrink much

A

Canada Balsam

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9
Q

At what degree of Incubation will the natural resin from the Canadian tree dissolve in Xylene?

A

37 C or 58C in paraffin oven

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10
Q

Refractive index of DPX

A

1.532

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11
Q

For small tissue sections only

A

DPX

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12
Q

Mounting media that preserves stains well

A

XAM

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13
Q

Refractive index of XAM

A

1.52

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14
Q

Resinous Mounting Media that is most commonly used, solidify within 20 mins

A

EUKITT

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15
Q

Made up of Gelatin, Glyserin jelly or gum Arabic

A

Aqueous mounting media

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16
Q

To prevent cracking and drying of prep

A

Glycerol

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17
Q

Used to increase refractive index

A

Sugar

18
Q

Refractive index of water

A
  1. 333
19
Q

Refractive index of Glycerin

A

1.46

20
Q

Aqueous mounting media for preservative action, sets hard, will keep sections mounted for years

A

Glycerin

21
Q

Standard mounting medium used when dehydration and clearing with xylene cannot be made

A

Glycerin jelly

22
Q

Prevents metachromatic stain to diffuse from the section

A

Fructose syrup

23
Q

Is readily available from outdated blood source stored in blood banks, dispensed into sterile tubes of 0.5 ml each

A

Plasma

24
Q

Added to slide before the application of coverslip for protection

A

Mounting medium

25
Q

The ratio of velocity of light in a vacuum to its velocity in a specified medium

A

Refractive index

26
Q

An aqueous detergent which can be purchased as a 0.1% solution

A

Poly-l-lysine

27
Q

Ratio of Poly-L-Lysine

A

1:10

28
Q

Best adhesive and better than Poly-L-Lysine

A

Apes

29
Q

Useful for cytology especially for cystospin preps of proteinacious or bloody material

A

Apes

30
Q

Most commonly used because it is easy to make, convenient and inexpensive

A

Mayer’s Egg Albumin

31
Q

Essential for methods that require exposure of sections to acids and alkalis during staining

A

Adhesive

32
Q

Ration of Mayer’s Egg Albumin

A

1:1

33
Q

Mayer’s Egg Albumin is made up of?

A

50ml egg white, 50ml glycerol, 100mg crystals of thymol

34
Q

Dried albumin is made up of?

A

5g dried albumin to 5g sodium chloride

35
Q

Best alternative adhesive

A

1% Gelatin

36
Q

Not necessary for routine staining, provided that the slides are clean and free from grease

A

Adhesive

37
Q

Gelatin-Formaldehyde mixture

A

5ml 1% Gelatin, 5ml 2% formaldehyde

38
Q

Good only for temporary mounting

A

Water

39
Q

Preferred over DPX and is soluble in xylene and is used as a 60% solution

A

Clarite/Clarite X

40
Q

Refractive index of Clarite/Clarite X

A

1.544