(5) Bilirubin

Question 1

Principle: Van den Berg reaction
* Performed at pH near 1.0

Answer 1

EVELYN & MALLOY

Question 2

Coupling accelerator for B1

Answer 2

Methanol

Question 3

o Diazo reagents for B2

Answer 3

✓ Diazo A: 0.1% sulfanilic acid + HCl
✓ Diazo B: 0.5% sodium nitrite
✓ Diazo blank: 1.5% HCl

Question 4

Final rxn for E&M:

Answer 4

o Pink to purple azobilirubin (w/ maximal absorption @ 560nm)

Question 5

Disadvantage of methanol:

Answer 5

causes ppt of proteins in
sample leading to turbidity

Question 6

• Popular technique for discreet analyzers (like architect etc.)
• More sensitive, thus, widely used
• Safer due to neutral pH

Answer 6

JENDRASSIK & GROFF

Question 7

Main reagent of JENDRASSIK & GROFF

Answer 7

Diazo reagent

Question 8

JENDRASSIK & GROFF accelerator

Answer 8

Caffeine Sodium Benzoate

Question 9

JENDRASSIK & GROFF buffer

Answer 9

Sodium acetate

Question 10

terminates the accelerator & destroys excess diazo reagent

Answer 10

Ascorbic acid:

Question 11

provides alkaline pH after
addition of ascorbic acid

Answer 11

Alkaline tartrate solution

Question 12

Final rxn JENDRASSIK & GROFF

Answer 12

blue azobilirubin (measured at 600nm)

Question 13

Ehrlich’s Reagent:

Answer 13

p-dimethylaminobenzaldehyde (p-
DABA)

1 Ehrlich unit = 1mg Urobilinogen

Question 14

Double Collection method (2 blood samples are collected)

Answer 14

ROSENTHAL WHITE method

Question 15

Spx collection for Rosenthal white

Answer 15

o 5mins after BSP administration
o 30mins after BSP administration

Question 16

BSP Dose for Rosenthal white

Answer 16

2mg/Kg body weight

Question 17

Single Collection method (only 1 blood sample is collected)

Answer 17

Mac Donald Method

Question 18

BSP Dose for Mac Donald Method

Answer 18

5mg/Kg body weight

Question 19

Spx collection for Mac Donald Method

Answer 19

o 45mins after BSP administration