Microscopes👩🏾‍🔬 Flashcards

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1
Q

What is the equation of magnification?

A

Size of image/real object size

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2
Q

What are the rules for a good scientific drawing?

A

-big enough not small
-dotted lines to show it is 3D
-labelled diagram
-titles
-state magnification in drawing
-no shades

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3
Q

What is the magnification?

A

Is the number of times a cell/object diminishes or enlarged

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4
Q

What is resolution?

A

Is the distance between 2 objects to be seen as separate for a clear visual.

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5
Q

How an optical light microscope work?

A

Light source(lamp) goes through 3 different lenses
-condenser lens
-specimen
-objective lens
-eyepiece lens

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6
Q

How to calibrate an eyepiece graticule?

A

Setup: Line up the eyepiece graticule scale to the stage micrometer scale
-record how many divisions of eyepiece graticule correspond to a know length of stage micrometer
-know length about stage micrometer/number of increments(lines) on eyepiece graticule.
Remember to convert all unit to micrometer meter

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7
Q

Does the eyepiece or stage micrometer move?

A

-Move:stage micrometer,10micro.m long
Divides in 100 sub-division
-Same/constant:eyepiece graticule
-10 micro.m long
-1micro.m subdivisions
-Smallest subdivision of 0.1 micro.m

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8
Q

What is the advantage + disadvantages of light microscope?

A

-portable
-easy to use
-can see dead or alive specimen
-cheap
Disadvantages
-lower resolution and magnification

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9
Q

What is the max resolution and magnification and what can’t it (light microscope)not view and how it forms images and focuses?

A

-Max resolution:0.2 micrometer
-Max magnification:*1500
-Can’t view: smaller organelles like mitochondria,ribosomes,ER,lysosomes.

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10
Q

Scale from cm to nm?

A

cm * 10= mm
mm * 1000= micrometer
Micrometer * 1000 = nm

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11
Q

What does light and T.E.M and S.E.M offer?

A

Light:gives a magnificated image and lower resolution and magnification
TEM:transmission electro microscope,sees inside,higher resolution and it is black and white
SEM:scanning electron microscope,3D view (structure is given but not inside)and higher resolution and magnification.

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12
Q

How does the optical light microscope form an image and how it makes it to focus?

A

Uses light to form image and focus uses glass lenses

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13
Q

How do we identify starch grains in plants cells?

A

Is the Iodine in
Potassium iodine solution to identify them

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