Light Microscopy And Resolution Flashcards
What is the thing at the top of the light microscope called
Eyepiece lens
What is the place where you put the specimen called
The stage
What is the thing called so you can focus on the object
Focusing dial
How much magnification is normally on a light microscope
X1000
What is different from the electron microscope
It can view living cells
Because it can see a living cell what does this mean it is able to see
Cell division
Movement of cells
What’s a disadvantage of light microscopy
Have to use a stain which can kill the cell
What is the definition of resolution
The minimum distance between two objects where they can still be seen as two different objects
What is the average resolution
200nm
Why is the resolution 200nm
Because of the nature of light
Wavelength of nature is light is 400-700nm
What happens if they are any closer than 200nm
You won’t be able to see them
