Elisa test

Question 1

why do we use the elisa test

Answer 1

to determine the quanitiy of the protien

Question 2

describe direct elsia
1- attach sample with potential ant…. to well
2- add complementary mono….. anti….. with enzy….. attched -> bind if present
3- w… well (remove unbound antibdies prev false pos)
4-add sub…..-> this allows the e……. to create the products that causes a c…. change (pos result)

Answer 2

antigen
monoclonal antibody
enzyme
wash
substrate
enzymes
colour

Question 3

indirect elisa
1- attach specific mo…….. ant…… to well
2- add sample with pot….. antig…… then w…. well
3-add complemetary mono…… ani….. with e…… attached -> bind an….. if presnt
4- w.. well -> removes un…. anti……
5- add sub….. ->en…… creates products that cause a c….. change (pos result)

Answer 3

monoclonal antibodies
potential antigens
wash
monoclonal antibodys
antigens
wash
unbound antigens
substrate
enzyme
colour