SYNTHESIS OF DNA

Question 1

Universal features of DNA replication

Answer 1

1. semi-conservative
2. specific base pairing
3. requirement for primers
4. 5’ to 3’ direction of replication
5. semi-discontinuous
6. origin of replication

Question 1

process wherein the original DNA strands are used as templates to synthesize new DNA strands

Answer 1

REPLICATION

Question 2

One parent molecule will replicate 2 molecule (each from the parent strand) and entirely new strand; These molecules will create another 2 molecules (bearing each from the parent strand) and entirely new strand) and another 2 molecules that is entirely new.

Answer 2

SEMI-CONSERVATIVE

Question 3

DNA replication is semi-conservative is demonstrated by ________________________________.

15^N medium —> 14^N medium —> 14^N
bacteria use the nitrogen to produce DNA molecule

Answer 3

MESELSON AND STAHL

Question 4

small segment to initiate the process of replication

Answer 4

REQUIREMENT FOR PRIMERS

Question 5

It is required as a primer to initiate the DNA synthesis

Answer 5

RNA segment

Question 6

the site of attachment of the next nucleotide

Answer 6

3’ OH

Question 7

Leading and lagging strands

Answer 7

SEMI-DISCONTINUOUS

Question 8

lagging strands

Answer 8

OKAZAKI FRAGMENTS

Question 9

Origin of replication

Answer 9

AT rich regions - have only 2 hydrogen bonds

Question 10

where enzymes and proteins penetrate to synthesize

Answer 10

DNA BUBBLES

Question 11

Collection of proteins and enzymes involved in the complex process of replication

Answer 11

REPLISOMES

Question 12

Direction of replication

Answer 12

5’ to 3’ direction

Question 13

Why are purines considered to have a greater mass than pyrimidines?

Answer 13

Because it has a double ring