Molecular Diagnostics Tutorial Flashcards

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1
Q

what is the appropriate way to take a sample from a child

A

swab of saliva or tooth surface

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2
Q

what organisms are important to detect in caries

A

strep mutans
lactobacillus species

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3
Q

what methods of isolating bacteria can be used

A

next generation sequencing
microbiome analysis of 16S rRNA

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4
Q

would knowing which bacteria and carriage levels of these bacteria impact overall levels of caries

A

yes because you can use it to monitor changes over time and can lead to change in public health measures

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5
Q

what preventive measures can be used to minimise bacterial carriage for caries

A

improved diet advice
behaviour advice
public health measures - fluoride

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6
Q

what specimens do you investigate for periodontal disease

A

subgingival plaque biofilm

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7
Q

where do you take periodontal disease microorganisms from

A

paper point from gingival crevice on site of inflammation

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8
Q

what organisms are important for periodontitis

A

p gingivalis
AA

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9
Q

what methods can be used to identify the culprit organism in periodontal disease

A

selective agars
PCR
NGS
grow on plates and undertake sensitivity testing

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10
Q

how do you determine cause and effect of periodontitis

A

removal of culprit organism leads to resolution of disease

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11
Q

what treatment options are available for periodontal disease

A

oral hygiene
antiseptics
antibiotics if unresponsive

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12
Q

what is the cause of inflammation associated with a denture

A

denture associated biofilm

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13
Q

what microorganisms are associated with denture stomatitis

A

candida albicans
candida glabrata

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14
Q

what types of specimens can be taken to isolate microorganisms associated with denture stomatitis

A

oral rinse or swab from localised area

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15
Q

why is it important to identify candidal species

A

c glabrata is insensitive to azoles

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16
Q

what are the systemic implications of an indwelling prosthesis for patients

A

aspiration pneumonia

17
Q

what are the microbiologically concerns for a patient with a facial swelling

A

are they systemically unwell?

18
Q

what type of clinical specimen should you take for someone with an abscess

A

take a pus sample so need a needle to aspirate it

19
Q

what type of microorganisms are you looking for in an abscess

A

oral anaerobes

20
Q

if you have an anaerobic species how would you take the specimen

A

be weary as they are oxygen sensitive so need anaerobic transport media

21
Q

what techniques are useful to identify microorganisms

A

standard plate culture and microscopy

22
Q

what are the systemic implications of an abscess if not dealt with

A

spreading odontogenic infections
possibility of sepsis