Genetic Fingerprinting Flashcards

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1
Q
A
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2
Q

What is gel electrophoresis used for?

A

to separate the VNTRs

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3
Q

What feature of DNA allows gel electrophoresis to work?

A

it is negatively charged and contains different lengths of VNTRs which are unique to individuals

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4
Q

What are 2 uses of genetic fingerprinting?

A
  • forensic analysis
  • parental disputes
  • breeding programmes
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5
Q

Why is DNA on the nylon membrane treated to form single strands?

A

so that DNA probes can attach/bind

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6
Q

List the order of the stages of genetic fingerprinting

A

Extraction
Digestion
Separation
Hybridisation
Development

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7
Q

Describe what happens in gel electrophoresis

A

DNA loaded in to wells
Buffer added
Charge passed through agar gel
DNA fragments move towards anode
Transferred to nylon membrane

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