Lab Exam 3 Cumulative Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Possible Slide Identifications:

A

Hydra

Hydra
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Possible Slide Identifications:

A

Planaria

Planaria
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Gram Staining

What are the steps to gram staining?

A
  1. Apply crystal violet
  2. Rinse off (with distilled water)
  3. Apply Grams iodine
  4. Rinse off
    5.Decolorize
  5. Rinse off
  6. Apply Safranin
  7. Rinse off and blot dry
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Gram Staining

What is the target cell when doing a differential gram stain? What is the nontarget cell?

A
  • Target cell = Gram Positive Bacteria
  • Non-target cell = Gram Negative Bacteria
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Temperature Experiment

What temperatures are refrigerator, room temp, and body temp?

In Celsius and Farinheit

A
  • Refrigerator: 4 degrees celsius
  • Room Temp: 25 degrees celsius
  • Body Temp: 37 degrees celsius
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Temperature Experiment

What is the equation to convert celisus to fahrenheit?

A

(X celsius multiplied by 9/5) + 32 = Fahrenheit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Bacterial Transformation

What genes are found on the pGLO plasmid?

A
  • B-lactamase gene
  • GFP gene
  • araC gene
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Bacterial Transformation

Which gene, when expressed, allows colonies to glow?

A

GFP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Bacterial Transformation

What bacteria did we use for this lab?

A

Escherichia Coli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Bacterial Transformation

What is needed in the media for the colonies to glow?

A

Arabinose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Antiseptic Lab

Which antiseptic is most effective? Which is least effective?

A
  • Most effective: Formaldehyde
  • Least effective: Isopropyl alcohol
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Antiseptic Lab

Which organisims were most and least resistant against antiseptics?

A
  • Most resistant: Escherichia coli
  • Least resistant: Mycobacterium smegmatis
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Antibiotic Lab

Which antibiotics were most and least effective?

A
  • Most effective: Chloramphenicol
  • Least effective: Colistin
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Antibiotic Lab

Which organisims were most and least resistant against antibiotics?

A
  • Most resistant = Escherichia coli
  • Least resistant = Mycobacterium smegmatis
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Selective and Differential Media

What are the original colors of each type of plate before the experiment starts?

A
  1. Eosin Methylene Blue: Dark purple
  2. Mannitol-Salt Agar: Red
  3. MacConkey Agar: Berry
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Selective and Differential Media

Which bacteria were gram + (used in MSA)?

A

Staphylococcus auerus, Staphylococcus epidermidis

17
Q

Selective and Differential Media

Which bacteria were gram - (used in EMB and MacConkey)?

A

Escherichia coli, Enterobacter aerogenes, Proteus vulgaris

18
Q

Selective and Differential Media

What would a strong fermenter EMB plate look like? Weak fermenter? No fermentation?

A
  • Strong Fermentation: Shiny Metallic Green
  • Weak Fermentation: Pink colonies around edges
  • No Fermentation: Plate remains purple
19
Q

Selective and Differential Media

What would a strong fermenter MacConkey Agar plate look like? Weak fermenter? No fermentation?

A
  • Strong Fermentation: Bright Pink
  • Weak Fermentaion: Light Pink
  • No Fermentation: Brown