The Practical Lab - QA/QC Flashcards

1
Q

What is important to check on machines to ensure results produced are reliable?

A
  • setup of the system
  • maintenace of analysers and system
  • interpretation of the results
  • checks carrier out to ensure that the values are accetable
  • accurate record keeping
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2
Q

What are the advatages of in-house labs?

A
  • fast turn around time
  • potential for improved monitoring
  • smaller volume of sample needed
  • available out of hours
  • available in more remote areas
  • may save costs
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3
Q

What factors affect results?

A

biological and analytical

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4
Q

What are the biological factors prior to sampling?

A

intral-individual and inter-individual

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5
Q

What are the inter-individual factors?

A

inherent differences between groups of animals due to effeects of species, breed, age and/or sex

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6
Q

What are haematology examples for species?

A

cat PCV is lower than dog PCV

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7
Q

What are haematology examples for breed?

A

akitas have a lower mean cell volume than other dogs

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8
Q

What haematology examples are there for age?

A

juvenile dogs have a lower PCV and TP than adult dogs

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9
Q

What haematology examples are there for the sex of a patient?

A

males usually have higher PCV values

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10
Q

What are the intra-individual factors?

A

often due to environmental/external factors such as diet, stress/excitement, reproductive status, drugs, method/site of blood samapling

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11
Q

What are the pre-analytical factors?

A

p- poor blood sampling technique
- haemolysed, lipaemic or icteric plsma
- wrong anticoagulation to blood ratio
- sterile vs non-sterile container
- transportation
- storage

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12
Q

How should samples be stored if not being tested immediately?

A

in the fridge

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13
Q

How should you package a sample for postal?

A
  • 50ml limit
  • sealed contianer
  • leakproof bag
  • absorbative padding
  • all packaged in rigid container
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14
Q

What are the analytical factors?

A
  • equipment - proper function
  • technician - correct training
  • analytical procedure - validated technique, reagents, colibrations
  • laboratoy - temperature, humidity
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15
Q

When is quality control performed?

A

during thw analysis to ensure the validity of the result

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16
Q

What should you do if a control failed?

A
  1. check obvious problems - reagent depletion/expiry, mechanical faults, clots
  2. use another aliquot (pot) of control materials
  3. repeat control
17
Q

If the control contnues to failure, what should you do?

A
  • use a new lot of reagent, recalibrate the equipment if possible and repeat control
  • run routine machine/instrument maintenance
  • consult manufacturer
18
Q

What post-analytical factors are there?

A
  • transfer of the results to patient record
  • archiving results
  • storing the specimen