exp 2.4 to investigate the effect of pH on enzyme activity

Question 1

substrate used in this exp

Answer 1

hydrogen peroxide (H2O2)

Question 2

enzyme

Answer 2

catalases

Question 3

source of the enzyme

Answer 3

celery

Question 4

how do we vary the pH

Answer 4

by using different pH buffers

Question 5

what is the variable in this exp

Answer 5

pH

Question 6

what is the constant in this experiment

Answer 6

temperature

Question 7

how do we keep the temperature constant

Answer 7

water bath

Question 8

what are the products of this exp

Answer 8

water + oxygen

Question 9

what is the function of washing up liquid in this experiment

Answer 9

traps oxygen bubbles as the reaction happens

Question 10

how do we measure the rate of enzyme activity

Answer 10

volume of foam produced per unit time

more foam produced=more enzyme activity

Question 11

draw the set up for this exp

Answer 11

water bath
test tube with H2O2
graduated cylinder with catalase, washing up liquid and pH buffer

Question 12

why are the substrate and enzyme kept seperate in the water bath

Answer 12

to prevent the reaction before the desired temperature of 25 degrees is reached

Question 13

how do you calculate the volume of foam produced

Answer 13

final volume - initial volume = volume of foam produced

Question 14

draw the graph of the results of this exp

Answer 14

y axis- rate of enzyme activity
x axis- pH
optimum activity at 7-9