exp 2.6 to investigate the effect of heat denaturation on enzyme activity Flashcards
1
Q
substrate
A
hydrogen peroxide
2
Q
enzyme
A
catalase
3
Q
source of enzyme
A
celery
4
Q
product
A
water and oxygen
5
Q
control
A
unboiled celery
6
Q
constant
A
pH
7
Q
how do we ensure pH stays constant
A
pH buffer of 7-9
8
Q
how do we denature the enzymes
A
boil celery/ heat above 60 degrees for 5 min
9
Q
what is the result of this exp
A
no foam is produced as the enzymes are denatured and can no longer function so there is no enzyme activity
10
Q
graph results
A
y axis= volume of foam produced
x axis = boiled vs unboiled catalase
11
Q
A
