exp 2.11 to isolate DNA from plant tissue

Question 1

what plant tissue did you use

Answer 1

onion/banana/kiwi

Question 2

how was the temperature kept constant

Answer 2

using a water bath beaker

Question 3

function of table salt

Answer 3

causes DNA molecules to clump together

Question 4

function of washing up liquid

Answer 4

helps dissolve nuclear membrane

Question 5

function of chopping into smaller pieces

Answer 5

increases surface area and breaks down cell wallsq

Question 6

why is the water bath at 60 degrees

Answer 6

to denature enzymes

Question 7

why do we put it in the beaker for exactly 15 min

Answer 7

to prevent damaging the DNA

Question 8

why do we cool the mixture

Answer 8

slows down the breakdown of DNA

Question 9

why do we blend

Answer 9

to break the cell wall and release DNA

Question 10

how long do we blend for

Answer 10

3 seconds

Question 11

function of coffee filter paper

Answer 11

lab filter paper pores are too small

Question 12

function of protease

Answer 12

enzyme that breaks down protein around DNA

Question 13

function of ice cold ethanol

Answer 13

DNA is insoluble in it

Question 14

how do we add the ice cold ethanol

Answer 14

down the side of the boiling tube

Question 15

what is the appearance of the DNA strands

Answer 15

white thread like structures

Question 16

why do we only blend for 3 seconds

Answer 16

so DNA is not destroyed