RP5 Enzymes Flashcards
1
Q
describe how you would investigate the effect of pH on the rate of reaction of the amylase enzyme
A
- place starch solution, amylase solution and buffer solution in a water bath at 25°C for a few minutes
- combine solutions and add a drop of the muxture to iodine solution on a tile
- add the mixture to iodine solution drops on the tile at fixed 30s intervalws until the iodine solution remains brown (does not turn blue-black)
- calculate the rate of reaction using 1/time taken for solution to remain brown
- repeat at different pH values using different buffer solutions
- plot graph of rate (y) against pH (x)
2
Q
which enzyme catalyses the breakdown of starch in the human digestive system?
A
amylase - catalyses breakdown of starch to maltose
3
Q
why must samples be left in the water bath before solutions are mixed?
A
to allow temperature to equilibrate
4
Q
what is a buffer solution?
A
a solution which can resist changes in pH
