Chapter 6/13: Cultivating Microbes on Media

Question 0

What is liquid media called?

Answer 0

Broth

Question 1

What is solid media called?

Answer 1

Agar

Question 2

What type of media has an unknown chemical composition?

Answer 2

Complex media

Question 3

What type of media has a precisely defined chemical composition?

Answer 3

Defined/complex media

Question 4

What type of specialized media allows for isolation of microbes with specific properties?

Answer 4

Selective media

Question 5

What type of specialized media allows certain microbes to be recognized based on visual reactions in the medium?

Answer 5

Differential media

Question 6

What type of specialized media can be used to increase a particular population of microbes with a specific property from a mixture of cell types?

Answer 6

Enriched media

Question 7

What is an example of a type of differential media?

Answer 7

MacConkey Agar (lactose fermentation)

Question 8

Which method(s) is/are qualitative for separating cells on an agar surface?

Answer 8

Streak plate method

Question 9

Which method(s) is/are quantitative for separating cells on an agar surface?

Answer 9

Spread plate method, Pour plate method

Question 10

What type of incubation method has bacterial colonies on surface of plate and in agar?

Answer 10

Pour plate method

Question 11

What type of incubation method has bacterial colonies on surface of plate?

Answer 11

Spread plate method

Question 12

What two types of cultivation independent methods can be used for identifying unculturable bacteria?

Answer 12

PCR, FISH (fluorescent in situ hybridization)