Flashcards in 1F Metabolic Pathways Deck (20):
What does an anabolic pathway require?
What does an anabolic pathway lead to?
The synthesis of a molecule.
What does a catabolic pathway release?
What does a catabolic pathway lead to?
The breakdown of a molecule.
Can catabolic and anabolic pathways have steps which are reversible and irreversible?
What are metabolic pathways controlled by?
Are genes for some enzymes continually expressed?
Most metabolic pathways are reversible. What can drive the sequence of reactions in a particular direction?
The presence of a substrate or the removal of a product.
Describe what is meant by an induced fit.
The enzymes active site changes shape to increase the contact between it and the substrate.
What is the roles of the active site
Orientate reactants, lower the activation energy of the transition state and the release of products with low affinity for the active side.
What is the effect of increasing enzyme concentration on the rate of a reaction?
The rate of the reaction increases until all substrate molecules are being acted upon.
What is the effect of increasing the substrate concentration on the rate of reaction?
Increases the rate of reaction until all active sites are accepted by substrate.
Do enzymes act in groups?
Can enzymes act as multi-enzyme complexes?
What is an inhibitor?
A substrate which reduces the rate of reaction.
What is a competitive inhibitor?
An inhibitor which had a similar shape to the substrate. It competes for the active site.
How does a competitive inhibitor work?
It binds to the active site and blocks it so that it cannot act upon the substrate.
How does a non-competitor work?
By binding to another region on the enzyme which is not the active site (allosteric site).
Describe end product inhibition.
End product inhibition is brought about by some of the end products of a reaction binding to an enzyme found at the beginning of the metabolic pathway and inhibiting the action of that enzyme.