2 miscellaneous gram nega bacilli .. Flashcards
(30 cards)
what species of gram negative bacilli uses RABBIT’S OR HORSE blood agar
a. haemophilus
b. helicobacter
c. staphylococcus
d. campylobacter
haemophilus
- RABBIT OR HORSE blood agar are commonly used for detecting hemolysis by hemolysin producing strains of haemophilus unable to grow in 5% SBA
- though they are “blood lovers”, most species cannot grow on pure BAP
x factor is produced by?
a. potato extract
b. yeast extract
c. breakdown of hemoglobin
X factor
- hematin/ protoporphyrin IX / hemin
- heat stable
- produced by the breakdown of hemoglobin
v factor is produced by
a. potato extract
b. yeast extract
c. breakdown of hemoglobin
V factor
- cofactor 1 or NAD
- heat labile
- produced by certain bacteria
- potato or yeast extract
which of the following species of haemophilus is the first bacterial genome to be sequenced and made public
a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus
haemophilus influenzae
- 1ST BACTERIAL GENOME TO BE SEQUUENCED AND MADE PUBLIC
which of the following species of haemophilus is the only xylose positive
a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus
haemophilus influenzae
-only xylose positive
agent of epiglottis
a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus
haemophilus influenzae
- PFEIFFERS BACILLUS
- agent of EPIGLOTTIS
which of the following haemophilus causes PINK EYE
a. haemophilus influenzae
b. haemophilus aegypticus
c. h. ducreyi
d. h. parahaemolyticus
haemophilus influenzae biogrp aegypticus
- causes pink eye. a very contagious conjunctivitis
- has a been isolated as the cause of BRAZILLIAN PURPURIC FEVER
which of the following serotype of haemophilus influenzae is most frequently encountered serotypes
a. haemophilus influenzae a
b. haemophilus influenzae b
c. haemophilus influenzae c
d. haemophilus influenzae d
e. haemophilus influenzae e
f. haemophilus influenzae f
HiB / haemophilus influenzae B
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which of the following on gram stain is school of fish
a. h. influenzae
b. h. aegyptcus
c. h. ducreyi
d. h. parainfluenzae
haemophilus ducreyi
- infective agent : CHANCROID. a soft chancre venereal disease
- requires addition growth factors and special media
- gram stain : school of fish
which of the following species can be mistaken as group a streptococcus on BAP
a. h. ducreyi
b. h. influenzae
c. h. haemolyticus
d. h. parainfluenzae
haemophilus haemolyticus
what media is preferred to use in haemophilus
a. cap
b. bap
c. thio
d. BHI
CAP is the preferred medium for Haemophilus because it contains X and V factor
rapid direct identification test for the capsular antigen of H. influenzae
a. porphyrin test
b. neufeld quallung reaction
c. cap
neufeld quallung reaction
- rapid direct identification test of the capsular antigen for H. influenzae
porphyrin test
- for identifying heme producing species of haemophilus
what is test reagent to be used in porphyrin test
a. horsed blod
b. rabbits blood
c. kovacs reagent
kovacs reagent ( p -dimethylaminobenzaldehyde)
which of the selective media to be used in H. influenzae for respiratory secretions of patients with cystic fibrosis
a. cap wiith 1% IsoVitaleX
b. nairobi biplate medium
c. horse’s blood bacitracin agar
h. influenzae - horse’s blood bacitracin agar
h. aegypticus - CAP with 1% IsoVitaleX or Vitox
h. ducreyi - sleective Nairobi biplate medium
which of the following organism is positive for X and v factor
i. h. influenzae
ii. h. aegypitus
iii. h. haemolyticus
iv. h. ducreyi
a. i, ii
b. ii only
c. i, ii, iii, iv
d. i, ii, iii
X and V {HAI} POSITIVE
h. HAEMOLYTICUS
h. AEGYPTICUS
h. INFLUENZAE
h. ducreyi is positive only on X factor
what spp uses WARTHIN STARRY SILVER STAIN for diagnosis
a. haemophilus
b. bartonella
c. afia
d. legionella
Bartonella
diagnosis : biopsy (warthin starry silver stain)
B. henselae : primary causative agent of CAT SCRATCH DISEASE
wihich of the following use reiterte’s stain
a. legionella
b. bartonella
c. bordetella
d. brucella
Legionella
isolated on enriched media : BCYE
stained by : REITERTE’S STAIN
which of the following spp uses BIPHASIC CASTANEDA BOTTLE for culture media
a. legionella
b. bartonella
c. bordetella
d. brucella
brucella
what is the screening test for early diagnosis of Legionella
a. culture
b. biopsy
c. urine ag test
d. motility test
e. string test
gold standard for identification of Legionella
- CULTURE (confirmatory)
Screening test for early diagnosis of Legionella
- urine antigen test (increase sensitivity)
which spp produces shiny mercury droplets on Regan Lowe media
a. legionella pneumophila
b. bartonella henselae
c. bordetella pertussis
d. brucella
bordetella pertussis
culture media:
+ Bordet gengou
+ Regan Lowe - selective (SHINY MERCURY DROPLETS )
which of the following spp can acquired through ingestion of contaminated animal products ( milk and meat)
a. legionella
b. bartonella
c. bordetella
d. brucella
brucella
- human can acquired this spp by eating contaminated animal products such as meats and milk
- agent of BRUCELLOSIS , UNDULANT FEVER AND MALTA FEVER
agent of pontiac fever
a. legionella pneumophila
b. legionella micdadei
c. legionella bozemanni
Legionella pneumophila
- agent of LEGIONNAIRE’S DISEASE (severe form)
- agent of PONTIAC FEVER (milder form)
- cooling towers for air conditioning and heating system
wiga’s agent of pneumophila
a. legionella pneumophila
b. legionella micdadei
c. legionella bozemanni
legionella bozemanni
- wiga’s agent of pneumophila
legionella micdadei
- pittsburg pneumonia agent
what stage of bordetella pertussis is bacterial multiplication
a. catarrhal
b. paroxysmal
c. convalescent
bordetella pertussis
three stages:
CATARRHAL - bacterial multiplication
PAROXYSMAL - violent and severe coughing
CONVALESCENT - last for 4 - 8 weeks
