preliminary biochemical test Flashcards
(23 cards)
ph indicator for glucose fermentation test
a. methyl red
b. phenol red
c. neutral red
glucose fermentation: indicator - PHENOL RED
positive result for carbohydrate fermentation test
a. red color
b. yellow
c. violet
carbohydrate fermentation test
positive result : pink - red –> YELLOW
mannitol / CHO: carbohydrtae fermentation test
Mangga - yellow
enzymes which breaks down hydrogen peroxide into water and oxygen
a. catalase
b. coagulase
b. cytochrome oxidase
catalase
what is the reagent used in catalase test
a. 30% h2o2
b. 3% h2o2
c. trytophan
3% h2o2
positive result in catalase
a. purple/ violet
b. effervescence
c. clotting
catalase : cupious bubbling / effervescence
coagulase : clotting / clumping
positive result in microdase test
a. blue
b. purple
c. yellow
microdase test : blue
cytochrome oxidase : purple / violet
recommended used in oxidase test to transfer the growth
a. iron containing wire
b. wooden sticks
c. platinum wire
oxidase test: if an IRON containing wire is used to transfer growth, a FALSE POSITIVE REACTION may result; therefore, PLATINUM WIRE OR WOODEN STICKS ARE RECOMMENDED
an enzyme which converts fibrinogen to fibrin
a. coagulase
b. catalase
c. cytochrome oxidase
coagulase
what kind of bacteria produce bound and free coagulase
a. s. pyogenes
b. s. aureus
c. streptococcus
s. aureus
gold standard plasma used in coagulate test
a. rabbit’splasma
b. human plasma
c. lysed rbc
rabbit’s plasma
if not available rabbit’s plasma human plasma in EDTA can be used / alternate
tests to differentiate Enterococcus and streptococcus
a. coagulase
b. catalase
c. spot indole tests
d. pyr hydrolysis
pyr hydolysis
what type of hemolysis is incomplete hemolysis
a. alpha
b. beta
c. gamma
d. alpha prime
alpha
- partial or incomplete lysis
- greenish or brownish color
what type of hemolysis has a brownish color
a. alpha
b. beta
c. gamma
d. alpha prime
alpha
- partial or incomplete lysis
- greenish or brownish color
what type of hemolysis has a wide zone of beta hemolysis surrounding the small zone of alpha hemolysis
a. alpha prime
b. beta prime
c. gamma
d. zeta
alpha prime
positive result for indole test
a. purple ring
b. red pink ring
c. clotting
red pink ring
what kind of microscope is when the specimen image appears dark against a bright background
a. brightfield
b. darkfield
c. phase contrast
brightfield microscopy
most critical step in performing gram stain procedure
a. primary stain
b. secondary stain
c. decolorizer
d. mordant
decolorizer
what is the primary stain to be used in acid fast stain
a. crystal violet
b. carbol fuschin
c. malachitie green
d. torgitol
acid fast stain
primary - carbol fuschin
mordant - steam heat/ phenol / torgitol
decolorizer - acid alcohol
secondary stain - methylene blue or malachite green
what to be decolorizer to be used in gram stain
a. acid alcohol
b. ethyl alcohol
gram stain - ethyl alcohol
acid fats stain - acid alcohol
what method on acid fast stain is cold method
a. zielh neelsen method
b. kinyoun method
ziehl neelsen - hot
kinyoun - cold (kold)
what is the positive control organism used in gram stain
a. staphylococcus aureus
b. staphylococcus pyogenes
c. e. coli
b & c
positive control : s. aureus and s. pyogenes
negative control : e. coli
what is the negative control organism used in gram stain
a. staphylococcus aureus
b. staphylococcus pyogenes
c. e. coli
e .coli
in afs what color is the result in secondary stain in non acid fast stain
a. blue
b. green
c. pink
d. red
if malachite green is used in secondary stain - green
while if methylene blue - blue
acid fast organism - red
