Flashcards in 23. Shigella Deck (25)
Disease caused by Shigella infection?
Bacillary dysentery (shigellosis)
3 species of Shigella that cause shigellosis?
S. dysenteriae, S. flexneri, S. sonnei
Shigella is a gram-______, ________cellular pathogen
Which other studied pathogenic bacteria is Shigella similar to? How?
Infectious dose of Shigella?
3 ways Shigella has features common to Salmonella/Listeria/pathogenic E.coli?
Uses T3SS effectors to control gap junctions
Causes active invasion
Causes membrane ruffling to invade host cells
What do Shigella hijack to cause the release of ATP from host cells?
Connexin 26 gap junction hemichannels
Purpose of causing ATP release?
Further increases bacterial invasion
What do Shigella use to control gap junctions?
Name of effector Shigella generates? What is it similar to?
ActA in Listeria
Purpose of IcsA?
Recruits actin to propel bacteria around cytoplasm (huge comet tails)
4 F's of the fecal-oral route?
Food, fingers, feces, flies
Where does Shigella replicate?
How does the shiga toxin function?
A subunit can bind to host ribosomes => inhibits protein synthesis
What can the shiga toxin cause to the host?
Watery diarrhea (w/ blood, mucus, pus)
Shigella is resistant to many _______
Is there a vaccine against Shigella?
Which lab animal model is not susceptible to Shigella? Which will develop shigellosis? Which gives similar disease as in humans?
Not susceptible = mice
Develop shigellosis = young guinea pigs
Similar to humans = monkeys
What part of cells do Shigella target for efficient cell-to-cell spreading?
What are tricellular junctions?
Junctions between multiple cells
Tricellular junctions use what protein to fuse adjacent membranes?
How does Shigella move from cell to cell?
Couples tricellulin identification and clathrin-mediated endocytosis
Shigella is a gram-_____, ____cellular pathogen
Is shigella aerobic or anaerobic?