Micro Lab Practicle

Question 0

What is room temp for incubation?

Answer 0

25C

Question 1

What is body temp for incubation?

Answer 1

37C

Question 2

Why is the position of the plate important?

Answer 2

To keep condensation from dripping and causing the bacteria to run together

Question 3

What materials are used in a grams stain?

Answer 3

Crystal violet, grams iodine, decolorizing alcohol, safranin red

Question 4

What does a grams stain tell us?

Answer 4

Morphology, positive or negative, arrangement

Question 5

Which stain do you not heat fix?

Answer 5

Negative stain

Question 6

What can a negative stain tell us?

Answer 6

Morphology only

Question 7

Whatdoes a spore stain do?

Answer 7

Helps determine if bacteria are spore formers

Question 8

What materials are used in a endosperm stain?

Answer 8

Malachite green, alcohol, and safranin red

Question 9

What is used in a negative stain?

Answer 9

India ink

Question 10

What is a capsule stain used for?

Answer 10

To determine if there is a capsule

Question 11

What do you use in a. Capsule stain?

Answer 11

India ink

Question 12

What is the trickiest but most important part of a gram stain?

Answer 12

Decolorizing alcohol

Question 13

What does counter stain mean?

Answer 13

Used to help differentiate different types of bacteria

Question 14

What is a mordant?

Answer 14

Locking mechanism

Question 15

What does gram variable mean?

Answer 15

Not sure if it is positive or negative, will be not quite purple or pink

Question 16

What is an acid fast stain good for?

Answer 16

Good for bacteria that have a lipid cell wall that doesn’t stain

Question 17

What is a bacterial capsule?

Answer 17

A gelatinous material coating the outside of the cell. A thick glycocalyx

Question 18

What is differential media?

Answer 18

Helps differentiate between bacteria, pos or neg, and what they favor

Question 19

What is selective media?

Answer 19

Prohibits or inhibits certain types of growth

Question 20

What is alpha growth?

Answer 20

Kinda used blood

Question 21

What is beta growth?

Answer 21

Used all blood

Question 22

What is gamma growth?

Answer 22

No blood

Question 23

What is osmolarity?

Answer 23

Bacterial growth in different concentrations of saline

Question 24

What is transduction?

Answer 24

Process in which phage infects bacteria

Question 25

What is meant by a lawn?

Answer 25

Evenly and adequately covering an area with bacteria

Question 26

What is a mueller Hinton agar?

Answer 26

Helps determine bacteriostatic or bacterialcidal

Question 27

What units to you use to measure the zones?

Answer 27

Mm

Question 28

Why would you preform a serial dilution exercise?

Answer 28

To lower microbe count to a countable level

Question 29

What does strepto mean?

Answer 29

Chains

Question 30

What does staphylo mean?

Answer 30

Bunches

Question 31

What does palisade mean?

Answer 31

Rods

Question 32

What does diplo mean?

Answer 32

Pairs

Question 33

What is the mueller Hinton agar?

Answer 33

Antibiotic sucrptibility

Question 34

What would you use a broth agar for?

Answer 34

Large number of bacteria

Question 35

What would you use a plate agar for?

Answer 35

Big surface area

Question 36

What would you use a slant agar for?

Answer 36

Smaller storage and it does not dry it as easy

Question 37

What would you use an agar deep for?

Answer 37

Decide if it is aerobic, anaerobic, or facultative

Question 38

What is a microorganism that an acid stain is good against?

Answer 38

Mycobacterium TB

Question 39

What is a vegetative cell?

Answer 39

An active cell

Question 40

What is a endo spore?

Answer 40

Inactive spore

Question 41

What color will a vegetative cell be?

Answer 41

Red

Question 42

What color will a endo spore be?

Answer 42

Green