counting/measuring microorganisms Flashcards

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1
Q

What are two direct methods of counting/measuring microorgansims?

A

plate count and membrane filteration

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2
Q

what is a plate count?

A

add measured amount of the sample to growth media, then count colonies that form

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3
Q

what is membrane filteration?

A

filter organisms onto membrane with a grid. you would count the organisms in a few grids and multiply by number of grids

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4
Q

what is a downside to using membrane filteration?

A

it counts the dead organsisms too

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5
Q

What are two indirect methods?

A

Turbidity and Luceferin

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6
Q

what is turbidity?

A

used in clear fluids, measures the cloudiness of sampl, but only works in high concentrations

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7
Q

what is luceferin?

A

it detects the presence of ATP produced by bacteria in foods or on surfaces

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8
Q

what is luceferin used for?

A

moniter sterility in food processing centers, operating rooms, etc.

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