gram positive or gram negative

Question 1

bacillus

Answer 1

gram positive rod

Question 2

Moraxella catarrhalis

Answer 2

gram negative cocci

Question 3

bacteroides

Answer 3

gram negative rod

enteric

Question 4

staphylococcus

Answer 4

gram positive cocci

Question 5

mycoplasma

Answer 5

no cell wall - sterols

these microbes may lack real colour - bc has no cell wall

Question 6

helicobacter

Answer 6

gram negative rod
enteric
silver stain like legionella. Legend of the Silver helicopter.

Question 7

klebsiella

Answer 7

gram negative rod

enteric

Question 8

listeria

Answer 8

gram positive rod

**food poisoning only one that is positive wee

Question 9

bacteroides

Answer 9

gram negative rod

enteric

Question 10

brucella

Answer 10

gram negative rod

zoonotic

Question 11

corynebacterium

Answer 11

gram positive rod

Question 12

neisseria

Answer 12

gram negative cocci

Question 13

haemophilus

Answer 13

gram negative rod
resp
pleomorphic (chylamydiae and rickettisiae)

Question 14

enterobacter

Answer 14

gram negative rod

enteric

Question 15

chlamydiae

Answer 15

gram negative
giemsa staining
pleomorphic
these microbes may lack real colour: intracellular parasite that lacks classic pepridoglycan because of low muramic acid

Question 16

pasteurella

Answer 16

gram negative rod

zoonotic

Question 17

proteus

Answer 17

gram negative rod

enteric

Question 18

yersinia

Answer 18

gram negative rod

enteric

Question 19

shigella

Answer 19

gram negative rod

enteric

Question 20

escherichiae

Answer 20

gram negative rod

enteric

Question 21

leptospira

Answer 21

gram negative spiral

Question 22

gardnerella

Answer 22

gram positive rod

GRAM VARIABLE

Question 23

clostridium

Answer 23

gram positive rod

Question 24

ureaplasma

Answer 24

no cell wall

contains sterols

Question 25

nocardia

Answer 25

gram positive
branching
weakly acid fast

Question 26

campylobacter

Answer 26

gram negative rod

enteric

Question 27

pseudomonas

Answer 27

gram negative rod

enteric

Question 28

bordetella

Answer 28

gram negative rod

respiratory

Question 29

francisella

Answer 29

gram negative rod

zoonotic

Question 30

rickettsiae

Answer 30

gram negative pleomorphic
giemsa (chlamydiae and borrelia)
these microbes may lack real colour: intracellular parasite

Question 31

mycobacterium

Answer 31

gram positive rod
acid fast
these microbes may lack real colour: high lipid content; mycolic acids in cell wall are detected by carbolfuchisin in acid fast stain

Question 32

salmonella

Answer 32

gram negative rod

enteric

Question 33

vibrio

Answer 33

gram negative rod

enteric

Question 34

borrelia

Answer 34

gram negative spiral
giemsa (with chlamydia, rickettsiae)
spiral with treponema and leptospira

Question 35

streptococcus

Answer 35

gram positive cocci

Question 36

lactobacillus

Answer 36

gram positive rod

Question 37

serratia

Answer 37

gram negative rod

enteric

Question 38

propionibacterium

Answer 38

gram positive rod

Question 39

leigonella

Answer 39

gram negative rod
respiratory
silver stain; these microbnes may lack real colour: primarily intracellular

Question 40

treponema

Answer 40

gram negative rod - DONT STAIN WELL bc are too thin to be visualized (these microbes may lack real colour - name them): dark-field microscopy and fluoresecent antibody staining
spiral (borrelia, leptospira)

Question 41

actinomyces

Answer 41

gram positive branching

Question 42

what microbes do not stain well?

Answer 42

these microbes may lack real colour
treponema - dark field
mycoplasma - no cell wall
mycobacteria - high lipid and mycolic acids that carbofuschin loves in acid fast
legionella - intracellular mostly - silver stain
ricketssiae - intracellular parasite
chlamydiae - intracellular parasite that lacks classic peptidoglycan becase of low muramic acid