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Microbiology - step > special stains > Flashcards

special stains Flashcards

1
Q

silver stain

A

legionella- gram negative rod, resp
fungi - pneumocystis
helicobacter pylori

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2
Q

legionella

A

silver stain

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3
Q

acid fast

A

mycobacterium carbofuschin (high lipid content and my;colic acids in cell wall)
nocardia
cryptosporidium oocytes.

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4
Q

nocardia

A

weakly acid fast

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5
Q

mycobacterium

A

carbofuschin in acid fast loves the mycolic acid in its high lipid content wall ;) :)

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6
Q

dark-field or fluorescent antibody staining

A

treponema

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7
Q

treponema

A

dark-filed ro fluorescent antibody staining.

hurrah.

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8
Q

giemsa

A 
chlamydia
rickettsia
borrelia
trypanosomes
plasmodium
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9
Q

chlamydia

pleopmorphic with BONUS what others?

A

giemsa
(rickettsia, borrelia, trypanosomes, plasmodium)
pleomorphic - chlamydia, rickettsia, haemophilus

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10
Q

rickettsia

pleomorphic with BONUS what others?

A

giemsa

  • (chlamydiae, borrelia, trypanosomes, plasmodium)
    pleopmorphic: chlamydia, rickettsia, haemophilus
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11
Q

borrelia

spiral with BONUS what others?

A

giemsa
(chlamydiae, rickettsia, trypanosomes, plasmodium)
spiral: borrelia, leptospira, treponema

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12
Q

trypanosomes

A

giemsa

*chlamydiae, rickettsia, borrelia, plasmodium)

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13
Q

plasmodium

A

giemsa

chlamydiae, rickettsia, borrelia, plasmodium

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14
Q

what does PAS stain?

A

Period-Acid-Schiff stain: staings GLYCOGEN, mucopolysaccharides

WHIPPLE DISEASE: tropheryma whipplei

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15
Q

tropheryma whipplei

A 
causes whipple disease
use PAS (periodic-acid-Schiff) to stain GLYCOGEN, and mucopolysaccharides.
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16
Q

Ziehl-Neelsen/carbolfuchsin

A

acid fast bacteria - nocardia, mycobacterium

protozoa: cryptosporidium oocyts

17
Q

cryptosporidium oocytes

A

protozoa stained with ziehl-Neelsen/carbolfuchsin

18
Q

india ink

A

cyrptococcus neoformans

19
Q

mucicarmine

A

Cryptococcus neoformans - thick capsule

20
Q

Cryptococcus neoforams

A

india ink

mucicarmine

21
Q

fungi like pneumocystis

A

silver stain

22
Q

helicobacter pylori

A

silver stain

Microbiology - step (9 decks)

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