L9

Question 1

Staphylococcus
Gram
motility
spores
oxygen

Answer 1

Gram +
Non motile
Non spore
facultative anaerobes

Question 2

Identify Staph

Answer 2

Catalase test(bubbles from h2o2)
Then see if grow in NaCl

Question 3

Coagulation test

Which strain is positive

Answer 3

Positive differentiates S. aureus

Question 4

Types of MSCRAMMS

Answer 4

Fibronectin binding protein

Collagen binding protein

Question 5

Pus

Answer 5

neutrophils

polymorphonuclear (PMNs)

Question 6

PMNs

Answer 6

first to show up

release ROS

Question 7

S aureus virulence factors

Answer 7

Polysaccaride capsule: block phagocytosis
Protein A: blocks antibody
Pore-forming toxins:Pop PMNs
-Panton-Valentine Leukocidin

Question 8

SSSS

Answer 8

Staphylcoccal scalded skin syndrome

Exfoliative toxins A and B disrupt desmosome junctions between outer 2 skin layers

Question 9

Toxic shock syndrom from S aureus is caused by

Answer 9

TSST-1

Question 10

TSST-1

Answer 10

Superantigen

Activate high levels of CD4 T cells

Question 11

IL1

Answer 11

Fever

Question 12

TNF-alpha and beta

Answer 12

hypotension, capillary leakage

Question 13

IFN-gamma, IL2

Answer 13

Rash

Question 14

Staphylococcal food poisoning

Answer 14

Enterotoxin A
Superantigens
Heat stable

Question 15

MRSA

Answer 15

Contains PBP2 allele

Penicillin cant bind to it

Question 16

VRSA

Answer 16

Vancomycin resistant s. arueus