Chapter 9: Enzymes Flashcards

1
Q

Shape of enzymes

A

Enzymes are 3D folded globular-shaped

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2
Q

What is an enzyme

A

An enzyme is a biological catalyst which is not used up in reactions are specific, e.g, amylase

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3
Q

Enzymes are used in

A

Digestion

Respiration

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4
Q

Denaturation

A

When an enzyme loses its shape and therefore its ability to function

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5
Q

Catalyst

A

Chemicals that change the rate of a reaction without being used up in the reaction itself

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6
Q

Specific

A

An enzyme’s ability to react with only one substrate, e.g, amylase acts on starch

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7
Q

Active site

A

The area of an enzyme where the substrate enters and is changed into a product

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8
Q

Substrate

A

The substance on which an enzyme acts on to break down into a product

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9
Q

Enzymes are influenced by

A

Temperature, eg, catalase at 25°
and
pH, eg, pepsin at pH 2 and catalase at pH 7

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10
Q

Optimum temperature for plant enzymes

A

25 - 30°

Not above 30°

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11
Q

Optimum temperature for animal enzymes

A

30 - 35°

Not above 37°

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12
Q

Bio-processing

A

The use of enzymes or any living cells or their components to useful products, e.g, yeast is used to make alcohol

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13
Q

Metabolism

A

The sum of all chemical reactions that occurs in an organism

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14
Q

Catabolism

A

A chemical reaction where larger molecules are broken down into smaller molecules
Digestion

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15
Q

Anabolism

A

A chemical reaction where smaller molecules are joined together to make larger molecules
Photosynthesis

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16
Q

Bio reactor

A

Vessel used for bio-processing

17
Q

Immobilized enzyme

A

Enzymes that are attached to or trapped in an inert insoluble material
Gel

18
Q

Physically immobilized

A

that Adsorption - attaching an enzyme to an inert insoluble material
Entrapment - trapping an enzyme in a gel or membrane

19
Q

Chemically immobilized

A

Attaching enzymes to each other and attracting charges parts of enzymes to charged parts of insoluble support to form covalent bonds

20
Q

Advantages of immobilized enzymes

A

Recovered easily
Easier to get a pure product
Efficiency is not affected

21
Q

Batch cultures

A

A cease with stirring and heating/ cooling system
which carry out product formation in individual cycles
Alcohol to

22
Q

Continuous cultures

A

A vessel used with stirring and heating/cooling systems and many sensors which maintain the correct conditions throughout the process
Lactose-free milk

23
Q

Advantages of batch culture

A

Product can be withdrawn before too many waste products build up

24
Q

Advantages of continuous culture

A

Sensors maintain correct conditions

pH, temperature, oxygen

25
Q

Disadvantages of batch culture

A

A lot of effort is required

Emptied, cleaned and sterilized more frequently

26
Q

Disadvantages of continuous culture

A

One contaminated product contaminats the rest

27
Q

Enzyme activity

A

Lipase in small intestine to break down lipids

Amylase in mouth to break down starch to maltose

28
Q

Test for alcohol

A

Clinistix turns from green to purple