Respiratory burst (oxidative burst)

Question 1

Enzyme that converts O2-* (superoxide anion) to H2O2

Answer 1

Superoxide dismutase

Question 2

Enzyme that converts H2O2 to HClO* (hypochloride)

Answer 2

Myeloperoxidase

Question 3

Enzyme that reduces GSSG to GSH

Answer 3

Glutathione reductase

Question 4

Enzyme that oxidizes GSH to GSSG and at the same time oxidizes NADPH to NADP+

Answer 4

Glutathione peroxidase

Question 5

NADPH oxidase deficiency leads to…

Answer 5

Chronic granulomatous disease (CGD)

Question 6

Organisms that can neutralize H2O2 are…

Answer 6

catalase positive

Question 7

Catalase positive organisms include…

Answer 7

Nocardia, Pseudomonas, Listeria, Aspergillus, Candida, E. coli, Staph, Serratia, B cepacia, H pylori

Question 8

What protein found in secretory fluids and neutrophils inhibits microbial growth via iron chelation

Answer 8

Lactoferrin

Question 9

What enzyme reduces NADP+ to NADPH

Answer 9

G6PD

Question 10

Blue-green heme-containing pigment that gives sputum its color

Answer 10

Meyloperoxidase

Question 11

What is the respiratory (oxidative) burst

Answer 11

Process that activates phagocyte NADPH oxidase complex which utilizes O2 as a substrate to generate ROS to kill bacteria