8.3 Flashcards
Transcription
The process of making RNA from DNA code
Central Dogma
DNA > RNA (transcription) > Protein (translation)
Base pairing rules
A to U and C to G (like DNA, but without the T)
Differences and similarities between DNA and RNA
RNA - Single stranded, has U instead of T.
DNA - Double stranded. Has T.
Both have A,C, and G.
Promoter
Starting site of transcription. Usually contains TATA. Signals transcription factors and then RNA polymerase attach.
Enhancer/silencer
Acts as a switch that controls if/how much RNA is made from gene. Additional binding for transcription factors.
Exons
Sequences that will be present in RNA
Introns
Sequences that will be spliced out and removed.
Initiation
Prepares RNA polymerase to begin copying gene sequence into mRNA.
Elongation
RNA breaks from transcription factors. 5’ protective cap is added to the RNA. Introns get spliced.
Termination
PolyA tail is added to the end of the RNA molecule. Longer tail = longer mRNA = more possible proteins.
What happens during the splicing process?
Introns are taken out of the RNA. Makes mature mRNA transcript.
Only happens in eukaryotes.
Alternative Splicing
Can create different proteins from the same gene.
It keeps different exons while different RNA sequences result in different protein structure/functions.