Intro to Microbiology

Question 1

What are the 5 categories of study in MMI 133?

Answer 1

Bacteriology, virology, mycology (fungi/yeast), parasitology (protozoa & metazoa) and immunology

Question 2

List the organisms we will be studying from largest to smallest.

Answer 2

Worms, bacteria, viruses and prions.

Question 3

What are the 3 major categories of bacteria?

Answer 3

Cocci, bacilli and spiral bacteria.

Question 4

What are the 4 different types of cocci bacteria?

Answer 4

Diplococci, staphylococci, streptococci and enterococci.

Question 5

What shape are cocci bacteria?

Answer 5

Round.

Question 6

What shape are bacilli bacteria?

Answer 6

Long, rod-shaped.

Question 7

What are the 3 different types of bacilli bacteria?

Answer 7

Diplobacilli, streptobacilli and coccobacilli.

Question 8

What are the 3 different types of spiral bacteria and what shapes are they?

Answer 8

Vibrio (comma-shaped), spirillum (wavy) and spirochete (tightly coiled).

Question 9

Describe how to do a Gram stain step-by-step.

Answer 9

1. Prepare slide with specimen, setting with heat or methanol.
2. Pour crystal violet over slide and wash off excess after 1 min.
3. Add iodine and wash off excess after 1 min.
4. Decolourize with a mix of acetone & alcohol until it runs clear, then wash with water.
5. Add safranin, wash off and dry slide.

Question 10

In the Gram stain, crystal violet is considered the _______ _____.

Answer 10

primary stain

Question 11

In the Gram stain, iodine is considered the _______.

Answer 11

mordant

Question 12

In the Gram stain, safranin is considered the ____________.

Answer 12

counterstain