JVD 2017 #2 - Comparison of Histological and Proliferation Features of Canine Oral Squamous Cell Carcinoma Based on Intraoral Location: 36 Cases

Question 1

What is PCNA, why is it important?

Answer 1

• PCNA (proliferating cell nuclear antigen) is a 36 kDa protein of DNA polymerase involved in cellular control of replication
• Overexpression related to prognosis and recurrence rate in OSCC in people

Question 2

What is Ki 67?

Answer 2

• Ki- 67 is a nuclear protein expressed in all cell cycle phases except G0
• Considered a proliferation marker

Question 3

What was the goal of the study?

Answer 3

to evaluate histological and proliferation difference for tumors based on intraoral location

Question 4

What proportion of tumors were located in each location?

Answer 4

• 36 total tumors
• 22 gingival (61%)
• 3 tongue (8%)
• 3 buccal mucosa (8%)
• 8 tonsillar (22%)

Question 5

What was found with respect to PCNA and Ki-67 staining with regard to tumor location?

Answer 5

•Neither Ki-67 nor PCNA PI was associated specifically with any of the intraoral locations evaluated

Question 6

What association was found between grade and location?

Answer 6

• •Grade of SCC associated with location
• no grade III in tongue or buccal mucosa (also no lymph node mets),
• more grade III in tonsil

Question 7

What association was found between PCNA Proliferation index and grade?

Answer 7

•PCNA PI showed significant Association with grade; lower PI found in grade I

Question 8

Which Marker was associated with lymph node metastases?

Answer 8

PCNA Proliferation index