JVD 2010 #2 - Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth Flashcards
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What was the methodology in this study?
radiographed and removed mandibular molars and premolars from 8 cats post euthanasia
demineralized and performed histopathology
performed immunolabelling for RANK, RANK-L and OPG
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What is RANK? What cells express RANK?
Receptor Activator of Nuclear Factor kB - type I membrane receptor
Expressed by dendritic cells, foreskin fibroblasts, some T-cells, and osteoclasts and their precursor cells
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What is RANKL? Where is it expressed?
•Recepto activator of nuclear factor kB - Ligand
expressed in lymphoid tissues and on leucocytes, osteoblasts, lymphocytes, and stromal cells
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What is OPG? Where does it come from? What does it do?
osteoprotegerin (OPG). It is secreted by osteoblasts and stromal cells in a soluble form as both a monomer and a dimer and acts as a decoy receptor for RANKL
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What was found with the immunolabelling in teeth undergoing resorption?
Teeth undergoing resorption usually displayed a strong staining with all three antibodies used, especially with anti- RANK and anti-RANKL antibodies, reflecting a high activity in alteration of tooth substances.
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What finding prompted the authors to conclude tresorption may be active in waves rather than continuously? What alternate explanation was there based on the M and M?
only 1/5 of the highly active resorptiove lesions contained odontoclasts.
Could have been lost in the demineralization process
According to David Senn, Dr med vet; Peter Schwalder, Dr med vet; Philippe Roux, Dr med vet; Dieter D. Bosshardt, PhD; Michael H. Stoffel; Dr Med vet
in
Immunohistochemical Localization of Osteoclastogenic Cell Mediators in Feline Tooth Resorption and Healthy Teeth
What was the overall conclusion of the study?
Study provides evidence for the occurrence of RANK, RANKL, and OPG in feline tooth resorption
