The lens through which you look to examine the slide.
Lenses with various powers of magnifications. Low: 4X Medium: 10X High: 40X Oil Immersion 100X
The surface on which the slide sits. Used to move the slide to the point of interest.
Supports the body of the microscope and typically houses the adjustment knobs.
Coarse Adjustment Knob
Large knob on the side of the arm and moves the stage up and down to change the distance of the stage from the objective lenses. Allows gross focusing of the image.
Fine Adjustment Knob
Allows fine-tuning of the image’s focus.
Also called the illuminator, provides the light source. It rests on the base of the microscope.
Adjustable wheel on the underside of the stage controls the amount of light allowed to pass through the slide.
Supports the eyepiece and the objective lenses.
A lens that serves to concentrate light from the illumination source that is in turn focused through the object and magnified by the objective lens.
Iris Diaphragm Lever
Part of the condenser that slides back and forth to control the opening that allows light through.
Metal piece under the glass in the ocular lenses.
Bending of light
An apparent increase in the size of the object.
Minimum distance between 2 objects that the microscope can distinguish as separate objects. (Resolution of the light microscope is 0.2 µm)
Ability to bring the image into clarity
Depth of Focus
Thickness within the specimen that is in sharp focus.
Quality of the microscope lenses that allows the focus to remain (about) the same when changing magnification.
Magnification of Ocular Lens
10X on to the objective lenses. (Low: 4X=40X; Medium: 10X=100X; High: 40X=400X; Oil Immersion: 100X=1000X)
Holding the Microscope
Use 2 hands to support the microscope when carrying it–one hand to hold the arm and the other hand to support the base.
Clean the lenses with lens paper only. Do not use paper towels or cloth to clean a lens, because this will scratch its surface.