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Clinical Microscopy > Amniotic Fluid > Flashcards

Amniotic Fluid Flashcards

(49 cards)

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1
Q

Major Volume Contributor after 1st Trimester

A

Feta Urine

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2
Q

Method of Collection

A

Amniocentesis

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3
Q

Specimen Collection

Method of Collection:
Volume:

A

Specimen Collection

Method of Collection: Amniocentesis
Volume: 30mL @ 14th week gestation

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4
Q

Fetal Assessment

Genetic Defects:
Fetal Lung Maturity:
HDN:

A

Fetal Assessment

Genetic Defects: 2nd Trimester - Dow Syndrome
Fetal Lung Maturity: 3rd Trimester
HDN: 3rd Trimester

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5
Q

Assessed in 3rd Trimester

A

FLM

HDN

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6
Q

Assessed in 2nd Trimester

A

Genetic Defects - Trisomy 21

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7
Q

Color Appearance

Colorless:
Yellow:
Green;
Red:
Dark Red Brown:
A

Color Appearance

Colorless: Normal
Yellow: HDN
Green: Meconium
Red: Traumatic Tap
Dark Red Brown: Fetal Death
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8
Q

Normal Color of Amniotic Fluid

A

Colorless

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9
Q

Color - HDN

A

Yellow

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10
Q

Color - Red

A

Traumatic Tap

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11
Q

Color - Dark Red Brown

A

Fetal Death

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12
Q

INCREASED Fetal Swallowing of Urine

A

OLIGOhydramnios

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13
Q

DECREASED Fetal Swallowing of Urine

A

POLYhydramnios

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14
Q

Quadruple tests prior to performing amniocentesis

A

AFP
Unconjugated Estriol
hCG
Inhibin A

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15
Q

Amniotic Fluid vs. Maternal Urine

Protein:
Glucose:
Creatinine:
Urea:

A

Amniotic Fluid

Protein: +
Glucose: +
Creatinine: <3.5mg/dL
Urea: <30 mg/dL

Maternal Urine

Protein: -
Glucose: -
Creatinine: >10 mg/dL
Urea: >300 md/dL

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16
Q

Specimen Handling

HDN:
FLM:
Cytogenetic Study:

A

Specimen Handling

HDN: Protected from Light
FLM: Iced
Cytogenetic Study: Room Temp (37C)

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17
Q

Anhydramnios

A

Decreased volume amniotic fluid in amniotic sac

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18
Q

Amniotic fluid is immediately protected from light to preserve _________

A

Bilirubin

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19
Q

Reference Method to Test for FLM

A

L/S ratio

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20
Q

Interpret:

L/S Ratio. >2.0

Study These Flashcards
A

Mature Fetal Lung

21
Q

Measured in Amniostat - FLM

Study These Flashcards
A

Phosphatidyl glycerol

22
Q

Positive Result - Foam Shake Test

Study These Flashcards
A

(+) Bubbles/Effervescence

23
Q

Foam Shake Test

Antifoaming agent:
Result:
Interpretation:

Study These Flashcards
A

Foam Shake Test

Reagent: 95% Ethanol
Result: Bubble
Interpretation: FLM

24
Q

Lamellar Body Count in Adequate FLM

Study These Flashcards
A

> /= 32, 000/uL

25
OD 650
Lamellar Body
26
Tests for HDN/Fetal Distress
Bilirubin
27
Test results for Fetal Distress (Bilirubin) is plotted in ___________
Liley Graph
28
Liley Graph Zone 1: None/Mild - ___________________ Zone 2: Moderately - _________________ Zone 3: Severely - _______________
Liley Graph Zone 1: None/Mild - No required intervention Zone 2: Moderately - Close Monitoring Zone 3: Severely - Requires Intervention - Exchange Transfusion
29
Danger Zone
Zone 2
30
Situation: Neonatal bilirubin exceeded 2nd zone Remedy: ________________
Requires Intervention
31
Situation: Neonatal bilirubin exceeded 1st zone Remedy: ________________
Close Monitoring
32
Test used to detect ruptured amnion
Fern Test
33
Fern Test Significance: Result:
Fern Test Significance: Ruptured amnion, Early Pregnancy Result: Fern-like Crystals
34
Neural Tube Defect Screening: Confirmatory:
Neural Tube Defect Screening: AFP - increased Confirmatory: Acetylcholinesterase - decreased
35
Neural Tube Defect Increased: Decreased:
Neural Tube Defect Increased: AFP Decreased: Acetylcholinesterase
36
Assess/Tests for Fetal Age
Creatinine
37
AFP is Increased in
Neural Tube Defect
38
AFP is Decreased in
Down Syndrome
39
OD 450
Bilirubin
40
Confirmatory Test for Neural Tube Defect
Acetylcholinesterase
41
Creatinine Level ``` Prior to 36 weeks gestation: 36 weeks (9 months): ```
Creatinine Level ``` Prior to 36 weeks gestation: 1.5 - 2.0 mg/dL 36 weeks (9 months): > 2.0 ```
42
9 month old - Creatinine Level
> 2.0
43
Pregnancy Testing - POCT ``` Specimen: Principle: Sensitivity: Detected: Anti-hCG source: ```
Pregnancy Testing - POCT ``` Specimen: First Morning Principle: Enzyme Immunoassay Sensitivity: 2-5 mIU/L Detected: B-hcg Anti-hCG source: Rabbit ```
44
Most specific for subunit for Hcg
Beta subunit
45
Hcg bioassays Male Frog: Female Frog:
Hcg bioassays Male Frog: Hogben Female Frog: Galli Mainini
46
Hcg peaks at _____________
1st trimester
47
Microviscosity is amniotic fluid is measured by _________
Flourescence Polarization
48
FLM in Microviscosity
>55 mg/grams of albumin
49
Internal Standard Microviscosity: L/S ratio:
Internal Standard Microviscosity: ALBUMIN L/S ratio: SPINGOMYELIN

Clinical Microscopy (15 decks)

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