Semen Analysis

Question 1

Site of Sperm Maturation

Answer 1

Epidydymis

Question 2

Source of High Zinc, ACP, and Proteolytic Enzyme

Answer 2

Prostate

Question 3

Secretes Fructose

Answer 3

Seminal Vesicles

Question 4

Produces Alkaline Urine

Answer 4

Bulbourethral Gland

Question 5

Specimen Collection

Abstinence:
Preferred Method:
Collected:

Answer 5

Specimen Collection

Abstinence: 2-3 days, not > 5 days
Preferred Method: Masturbation
Collected: ENTIRE EJACULATE

Question 6

In collecting seminal fluid, _______________ should be collected

Answer 6

ENTIRE EJACULATE

Question 7

Semen analysis should be done only after ____________

Answer 7

Liquefaction

Question 8

If not processed immediately, specimen should be kept at ______

Answer 8

37C

Question 9

Absence of sperm cells

Answer 9

Azoospermia

Question 10

No Ejaculate

Answer 10

Aspermia

Question 11

Appearance

Normal:
Infection:
Red/Brown:
Yellow:

Answer 11

Appearance

Normal: Graysih white, tranluscent
Infection: Increased white turbidity
Red/Brown: Presence of Blood
Yellow: Prolonged Abstinence

Question 12

Color - Prolonged Abstinence

Answer 12

Yellow

Question 13

Yellow discoloration is due to __________________

Answer 13

INCREASED FLAVIN

Question 14

Grading - Watery

Answer 14

0

Question 15

Grading - Gel like

Answer 15

4

Question 16

Normal Semen Volume

Answer 16

2-5 mL

Question 17

Normal Semen

Liquefaction Time:
Color:
Viscosity:
Odor:
pH:
Volume:
Motility:

Answer 17

Normal Semen

Liquefaction Time: 30-60 minutes
Color: Grayish white
Viscosity: pour in droplets
Odor: Clorox-like
pH: 7.2 - 8.0
Volume: 2-5 ml
Motility: Grade 2

Question 18

pH

Answer 18

7.2 - 8.0

Question 19

Effect of Increased prostatic fluid

Answer 19

DECREASED pH

Question 20

Effect of Prolonged Abstinence
1.
2.
3.

Answer 20

Increase Volume
Decrease Motility
Yellowish Color

Question 21

Specimen should be delivered to the lab within ____ of collection @Room Temp

Answer 21

1 hr

Question 22

Result if part of the FIRST portion is Missing

Increased:
Decreased:
Specimen will not ______.

Answer 22

Result if part of the FIRST portion is Missing

Increased: pH
Decreased: Sperm Count
Specimen will not LIQUEFY.

Question 23

Result if part of the LAST portion is Missing

Increased:
Decreased:
Specimen will not ______.

Answer 23

Result if part of the LAST portion is Missing

Increased: Sperm count
Decreased: Volume, pH
Specimen will not CLOT

Question 24

Sperm Motility Grading

4 - Rapid, \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
3 - \_\_\_\_\_\_\_\_\_, Lateral Movement
2 - \_\_\_\_\_\_\_\_\_, \_\_\_\_\_\_\_\_\_\_\_
1 -  \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
0 - \_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_

Answer 24

Sperm Motility Grading

4 - Rapid, Straight Line
3 - Slower, Lateral Movement
2 - Slow Forward, Noticeable Lateral Movement
1 - No Forward Progression
0 - No movement at all

Question 25

Used Counting Chamber for UNDILUTED SPECIMEN

Answer 25

Makler Counting Chamber

Question 26

Diluting Fluid in Specimen Analysis

Answer 26

NSS or Cold Tap Water

Question 27

NV - Sperm Motility

Answer 27

>50% within 1 hr

Question 28

In sperm morphology, evaluate at least ______ sperm cells

Answer 28

200 sperm cells

Question 29

Sperm Morphology ``` Routine Criteria: Strict Criteria (Kruger's): ```

Answer 29

Sperm Morphology ``` Routine Criteria: >30% normal morphology Strict Criteria (Kruger's): >14% normal morphology ```

Question 30

Stain of Choice for Evaluating Sperm Morphology

Answer 30

Papanicolaou's

Question 31

Immature Sperm Cell

Answer 31

Spermatid

Question 32

Most common cause of Male Infertility

Answer 32

Varicocele

Question 33

Seminal Fluid FRUCTOSE is tested w/in ____ hours or _________ to prevent fructolysis

Answer 33

2 hours | Frozen

Question 34

Screening Test for Fructose

Answer 34

Resorcinol Test

Question 35

Positive Result - Resorcinol Test

Answer 35

Orange Red Color

Question 36

Test for Sperm Viability

Answer 36

Modified Bloom's Test

Question 37

Modified Bloom's Test Reagent: ________________ (+) Living Sperm: ____________

Answer 37

Modified Bloom's Test Reagent: Eosin-Nigrosine Stain (+) Living Sperm: Unstained, Bluish white

Question 38

Test for Choline

Answer 38

Florence Test

Question 39

Florence test (nonspecific) Tests for: _________ Rgt: __________ Result: (+) ______________

Answer 39

Florence test (nonspecific) Tests for: CHOLINE Rgt: Iodine crystals + KI Result: (+) Dark brown rhombic crystals

Question 40

Positive Result - Florence Test

Answer 40

(+) Dark brown rhombic crystals

Question 41

Test for Spermine

Answer 41

Barbiero’s test

Question 42

Barbiero’s test (specific) Tests for: _________ Rgt: __________ Result: (+) ______________

Answer 42

Barbiero’s test (specific) Tests for: SPERMINE Rgt: Sat. Picric Acid +TCA Result: (+) Yellow leaf-like crystals

Question 43

Positive Result - Barbiero’s test

Answer 43

(+) Yellow leaf-like crystals