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ANALYTICAL METHOD PT 2 Flashcards

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1
Q

IS THE MIGRATION OF CHARGED PARTICLES AND IT SEPARATES PROTEINS ON THEIR ELECTRIC CHARGE AND DENSITIES

A

ELECTROPHORESIS

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2
Q

DURING ELECTROPHORESIS WHAT HAPPENS TO THE PROTEIN

A

NEGATIVELY CHARGED (ANIONS) AND MOVED TOWARDS THE ANODE

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3
Q

WHAT ARE THE (5) COMPONENTS OF ELECTROPHORESIS

A

ELECTRICAL POWER

SUPPORT MEDIUM

BUFFER

SAMPLE

DETECTOR BUFFER ( BARBITAL pH8.6 )

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4
Q

TERM USED FOR IT HAS NET CHARGE THAT CAN BE EITHER POSITIVE OR NEGATIVE DEPENDING ON pH CONDITIONS

A

AMPHOTERIC

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5
Q

TERM USED FOR THE MOVEMENT OF BUFFER IONS AND SOLVENT RELATIVE TO FIXED SUPPORT

A

ENDOSMOSIS

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6
Q

TERM USED FOR THE MIGRATION OF SMALL CHARGED IONS

A

IONTOPHORESIS

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7
Q

TERM USED FOR THE MIGRATION OF CHARGED MACROMOLECULES

A

ZONE ELECTROPHORESIS

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8
Q

(5) FACTORS AFFECTING RATE OF MIGRATION

A

NET ELECTRIC CHARGE OF THE MOLECULE

SIZE AND CHARGE OF THE MOLECULES

ELECTRIC FIELD STRENGTH

NATURE OF THE SUPPORTING MEDIUM

TEMPERATURE OF OPERATION

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9
Q

WHAT ARE THE (3) SUPPORTING MEDIA

A

CELLULOSE ACETATE

AGAROSE GEL

POLYACRYLAMIDE GEL

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10
Q

WHICH SUPPORTING MEDIA SEPARATES BY MOLECULAR SIZE

A

CELLULOSE ACETATE

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11
Q

WHICH SUPPORTING MEDIA SEPARATES BY ELECTRICAL CHARGE AND DOS NOT BIND THE PROTEIN

A

AGAROSE GEL

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12
Q

WHICH SUPPORTING MEDIA SEPARATES ON THE BASIS OF CHARGE AND MOLECULAR SIZE AND IT IS ALSO USED TO STUDY_______

A

POLYACRYLAMIDE GEL

ISOENZYMES

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13
Q

STAIN USED FOR VISUALIZATION OF FRACTION BANDS OF (PROTEIN)

A

AMIDO BLACK

PONCEAU S

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14
Q

STAIN USED FOR VISUALIZATION OF FRACTION BANDS OF ( FATS)

A

OIL RED O

SUDAN BLACK

FAT RED 7B

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15
Q

STAIN USED FOR VISUALIZATION OF FRACTION BANDS OF ( CSF PROTEIN )

A

COOMASSIE BLUE

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16
Q

STAIN USED FOR VISUALIZATION OF FRACTION BANDS THAT IS VERY SENSITIVE TO NANOGRAM QUANTITIES OF PROTEINS

A

GOLD/SILVER STAIN

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17
Q

IT MEASURES THE ABSORBANCE OF STAIN

A

DENSITOMETRY

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18
Q

WHAT METHOD SEPARATES MOLECULES BY MIGRATION THROUGH pH GRADIENT

IT IS ALSO IDEAL FOR SEPARATING PROTEINS OF IDENTICAL SIZES WITH DIFFERENT NET CHARGES

A

ISOELECTRIC FOCUSING

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19
Q

(4) ADVANTAGES OF ISOELECTRIC FOCUSING

A

ABILITY TO RESOLVE MIXTURE OF PROTEINS

TO DETECT ISOENZYMES OF ACP, CK AND ALP IN SERUM

TO IDENTIFY GENETIC VARIANTS OF PROTEINS SUCH AS ALPHA-1-ANTITRYPSIN

TO DETECT CSF OLIGOCLINAL BANDING

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20
Q

WHAT METHOD IS SAMPLE MOLECULES ARE SEPARATED BY ELECTRO-OSMOTIC FLOW (EOF)

A

CAPILLARY EPECTROPHORESIS

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21
Q

(3) USES OF CAPILLARY ELECTROPHORESIS

A

SEPARATION

QUANTITATION

ANALYSIS OF PROTEIN PRODUCTS

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22
Q

WHAT METHOD INVOLVES THE SEPARATION OF SOLUBLE COMPONENTS

A

CHROMATOGRAPHY

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23
Q

(6) BASES OF SEPARATION IN CHROMATOGRAPHY

A

RATE OF DIFFUSION

SOLUBILITY OF THE SOLUTE

NATURE OF THE SOLVENT

SAMPLE SOLUBILITY

MOLECULAR SIZE

IONIC ATTRACTION

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24
Q

(2) FORMS OF CHROMATOGRAPHY

A

PLANAR

COLUMN

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25
CHROMATOGRAPHY METHOD USED FOR FRACTIONATION OF SUGAR AND AMINO ACID IT USES WHAT SORBENT
PAPER CHROMATOGRAPHY WHATMAN PAPER
26
METHOD FOR SEMIQUANTITATIVE DRUG SCREENING TEST IT USES WHAT SORBENT
THIN LAYER CHROMATOGRAPHY THIN PLASTIC PLATES IMPREGNATED WITH LAYER OF SILICA GEL
27
BIOLOGICAL SAMPLES THAT CAN BE USED IN TLC (3)
BLOOD URINE GASTRIC FLUID
28
METHOD USED FOR SEPARATION OF STEROIDS, BARBITURATES, BLOOD, ALCOHOL AND LIPIDS ALSO USEFUL FOR COMPOUNDS THAT ARE NATURALLY VOLATILE
GAS CHROMATOGRAPHY
29
SAMPLES ARE INTRODUCED INTO GAS CHROMATOGRAPHY USING
HYPODERMIC SYRINGE / AUTOMATED SAMPLER
30
USED AS A DETECTOR FOR GAS LIQUID CHROMATOGRAPHY (GLC)
FLAME IONIZATION
31
METHOD OF SEPARATION OCCURS BASED ON DIFFERENCES IN ABSORPTION AT SOLID PHASE SURFACES
GAS SOLID CHROMATOGRAPHY
32
METHOD OF SEPARATION OCCURS BY DIFFERENCES IN SOLUTE PARTITIONING BETWEEN GASEOUS MOBILE PHASE AND LIQUID STATIONARY PHASE
GAS LIQUID CHROMATOGRAPHY
33
METHOD IS BASED ON THE FRAGMENTATION AND IONIZATION OF MOLECULES
MASS SPECTROSCOPY
34
THIS METHOD IS THE GOLD STANDARD FOR DRUG TESTING AND IT IS QUANTITATIVE MEASUREMENT OF DRUG CAN BE PERFORMED
GAS CHROMATOGRAPHY- MASS SPECTROSCOPY
35
GC-MS IS ALSO USED FOR (3)
XENOBIOTICS ANABOLIC STEROIDS PESTICIDES
36
METHOD THAT CAN DETECT 20 INBORN ERRORS OF METABOLISIM FROM A SINGLE BLOOD SPOT
TANDEM MASS SPECTROSCOPY
36
DISTRIBUTION OF SOLUTES BETWEEN A LIQUID MOBILE PHASE AND STATIONARY PHASE
LIQUID CHROMATOGRAPHY
37
WHAT IS THE MOST WIDELY USED LIQUID CHROMATOGRAPHY
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
38
METHOD THAT USES PRESSURE FOR FAST SEPARATIONS IT IS ALSO USED IN (2)
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY FRACTIONATION OF DRUGS, HORMONES, LIPIDS, CARBOHYDRATES AND PROTEINS & RAPID HbA1c TEST WITHIN 5 MINS
39
METHOD FOR DETECTING NONVOLATILE SUBSTANCES IN BODY FLUIDS TO CONFIRM POSITIVE RESULTS FROM SCREENING OF ELICITED DRUGS COMPLEMENTARY METHOD FOR GC-MS
LIQUID CHROMATOGRAPHY- MASS SPECTROSCOPY (LC-MS)
40
IT IS USED IN HPLC AND GC METHODS TO COMPENSATE
INTERNAL STANDARD
41
TYPE OF CHROMATOGRAPHY THAT IS EXCHANG OF SAMPLE IONS USED FOR SEPARATION OF AMINO ACIDS, PROTEINS AND NUCLEIC ACIDS
ION EXCHANGE CHROMATOGRAPHY
42
TYPE OF CHROMATOGRAPHY USED FOR SEPARATION OF THERAPEUTIC DRUGS AND THEIR METABOLITES
PARTITION CHROMATOGRAPHY
43
TYPE OF CHROMATOGRAPHY THAT USES IMMOBILIZED BIOCHEMICAL LIGANDS USED FOR SEPARATION OF LIPOPROTEINS, CARBOHYDRATES AND GLYCATED HEMOGLOBINS AND ANTIBODIES
AFFINITY CHROMATOGRAPHY
44
TYPE OF CHROMATOGRAPHY THAT SEPARATION IS BASED ON DIFFERENCES BETWEEN ADSORPTION AND DESORPTION OF SOLUTES
ADSORPTION CHROMATOGRAPHY
45
METHOD THAT MEASURES THE AMOUNT OF LIGHT INTENSITY OVER A ZERO BACKGROUND
FLUOROMETRY
46
THE PRINCIPLE OF THIS METHOD IT DETERMINES THE AMOUNT OF LIGHT EMITTED BY A MOLECULE AFTER EXCITATION
FLUOROMETRY
47
LIGHT DETECTOR USED IN FLUOROMETRY: LIGHT SOURCE USED IN FLUOROMETRY: MONOCHROMATOR USED IN FLUOROMETRY
PHOTOTUBE XENON LAMP & MERCURY ARC LAMP 2 MONOCHROMATORS ( EITHER PRISMS, FILTER, GRATINGS)
48
THIS METHOD IS ABOUT 1000X MORE SENSITIVE THAN SPECROPHOTOMETER IT IS AFFECTED BY QUENCHING
FLUOROMETRY
49
PRINCIPLE OF THIS METHOD IS THE CHEMICAL REACTION YIELDS AN ELECTRONICALLY EXCITED COMPOUND THAT EMITS LIGHT AS IT IS GROUND STATE
CHEMILUMINESCENCE
49
THIS METHOD IS THE EMISSION OF LIGHT IS CREATED FROM A CHEMICAL OR ELECTROCHEMICAL REACTION IT IS MORE SENSITIVE THAN FLUORESCENCE
CHEMILUMINESCENCE
50
CHEMILUMINESCENCE IS ALSO USED IN
IMMUNOAASAYS
51
PHOTODETECTOR USED IN CHEMILUMINESCENCE
PHOTOMULTIPLIER TUBE
52
MEASUREMENT OF OSMOLALITY OF AN AQUEOUS SOLUTIONS SUCH AS SERUM, PLASMA , AND URINE.
OSMOMETRY
53
PRINCIPLE OF THIS METHOD IS BASED ON MEASURING CHANGES IN COLLIGATIVE PROPERTIES OR SOLUTIONS
OSMOMETRY
54
OSMOTIIC PARTICLES (3)
GLUCOSE UREA NITROGEN SODIUM
55
COLLIGATIVE PROPERTIES OF SOLUTION (4)
OSMOTIC PRESSURE BOILING POINT FREEZING POINT VAPOR PRESSURE
56
MOST COMMONLY USED METHOD FOR MEASURING THE CHANGES IN COLLIGATIVE PROPERTIES OF SOLUTION
FREEZING-POINT DEPRESSION OSMOMETRY
57
MEASUREMENT OF CURRENT OR VOLTAGE
ELECTROCHEMISTRY TECHNIQUES
58
MEASUREMENT OF ELECTRICAL POTENTIAL DUE TO FREE IONS ALSO A MEASUREMENT OF DIFFERENCES IN VOLTAGE ANS CONSTANT CURRENT
POTENTIOMETRY
58
POTENTIOMETRY FOLLOW THE EQUATION OF
NERNST EQUATION
59
REFERENCE ELECTRODES OF POTENTIOMETRY (2)
CALOMEL SILVER-SILVER CHLORIDE
60
POTENTIOMETRY USES (3)
pH PCO2 Tests
61
ELECTROCHEMICAL TRANSDUCER CAPABLE OF RESPONDING TO ONE GIVEN ION ITIS VERY SENSITIVE AND SELECTIVE FOR THE ION IT MEASURES
ION SELECTIVE ELECTRODE
62
MEASURE THE ELECTROLYTE DISSOLVED IN FLUID PHASE OF SAMPLE IN MMOL/L OF PLASMA WATER
ISE ANALYZER
63
64
(2) TYPES OR ION SELECTIVE ELECTRODE (ISE)
DIRECT ISE ( WITHOUT SAMPLE DILUTION) INDIRECT ISE ( WITH SAMPLE DILUTION)
65
ISE MEMBRANE (3)
GLASS ALUMINUM SILICATE ( SODIUM ) VALINOMYCIN GEL ( POTASSIUM) ORGANIN LIQUID MEMBRANE ION EXCHANGERS (CALCIUM AND LITHIUM)
66
MEASUREMENT OF THE AMOUNT OF ELECTRICITY AT A FIXED POTENTIAL IT FOLLOW_______
COULOMETRY FARADAY’S LAW
67
COULOMETRY USES ______ in CSF,SERUM AND SWEAT
CHLORIDE TEST
68
COULOMETRY INTERFERENCE (3)
BROMIDE CYANIDE CYSTEINE
69
MEASUREMENT OF THE CURRENT FLOW PRODUCED BY AN OXIDATION-REACTION
AMPEROMETRY
70
AMPEROMETRY USES (4)
pO2 GLUCOSE CHLORIDE PEROXIDASE DETERMINATION
71
MEASUREMENT OF DIFFERENCE IN CURRENT AT A CONSTANT VOLTAGE IT FOLLOW _____EQUATION
POLAROGRAPHY ILKOVIC
72
MEASUREMENT OF CURRENT AFTER WHICH A POTENTIAL IS APPLIED TO ELECTROCHEMICAL CELL
VOLTAMMETRY
73
FOR LEAD AND IRON
ANODIC STRIPPING VOLTAMMETRY

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