LAB APPARATUSES & INSTRUMENTS

Question 1

USED TO SEPARATE PARTICLES SUSPENDED IN A LIQUID ACCORDING TO ______,_____,_______ AND ______.

Answer 1

CENTRIFUGE

PARTICLE SIZE, DENSITY, VISCOSITY OF MEDIUM AND ROTOR SPEED

Question 2

CAUSE PARTICLES OF HIGHER DENSITY THAN SOLVENT TO SINK

WHAT WILL HAPPEN TO THOSE LESS THAN SOLVENT

Answer 2

GRAVITATIONAL FORCE

FLOAT ON THE TOP OF TUBE

Question 3

2 TYPES OF CENTRIFUGE

Answer 3

SWINGING BUCKET

FIXED ANGLE

Question 4

2 TYPES OF ROTOR

Answer 4

SWING OUT ROTOR (HORIZONTAL PELLET)

FIXED ANGLE ROTOR (PARALLEL/SLANT PELLET)

Question 5

HOW DO YOU CHOOSE A CENTRIFUGE

Answer 5

CENTRIFUGE SPEED

CENTRIFUGE SIZE

Question 6

RCF STANDS FOR?

FORMULA OF RCF:

Answer 6

RELATIVE CENTRIFUGAL FORCE

RCF= 1.118 X 10^-5 X r X rpm^2

Question 7

2 CENTRIFUGE SIZE

Answer 7

FLOOR- STANDING

TABLE- TOP

Question 8

SAFETY PRECAUTIONS WHEN WORKING WITH CENTRIFUGE

Answer 8

ENSURE A STURDY, LEVEL WORKSURFACE

BALANCE THE CENTRIFUGE

DO NOT OPEN THE LID WHILE ROTOR IS MOVING

IF CENTRIFUGE IS WOBBLING/SHAKING PULL THE PLUG

Question 9

HOW DO YOU BALANCE A CENTRIFUGE

Answer 9

ENSURE ALL SAMPLE TUBES ARE EVENLY FILLED, FILL THE TUBES WITH WATER TO ENSURE THE MASS IS BALANCED TO 0.1 grams

PLACED OTHER TUBES DIRECTLY OPPOSITE TO ENSURE THE CENTER OF GRAVITY

ROTATE THE ROTOR 90 DEGREE

REPEAT

Question 10

WHAT ARE THE (10) TYPES OF PIPPETES

Answer 10

SEROLOGICAL PIPETTE

MOHR PIPETTE

VOLUMETRIC PIPETTE

OSTWALD FOLIN PIPETTE

PLASTIC DISPOSABLE PIPETTE

AUTOMATIC PIPETTE

MULTI CHANNEL PIPETTE

AIR DISPLACEMENT PIPETTE

POSITIVE DISPLACEMENT PIPETTE

DILUTOR PIPETTE

Question 11

WHAT IS THE (2) PIPETTING TECHNIQUE

Answer 11

MENISCUS IS ABOVE THE GRADUATION LINE

BOTTOM OF MENISCUS

Question 12

STEPS OF USING GLASS PIPETTE

Answer 12

USING MECHANICAL SUCTION

WIPE OFF OUTSIDE OF PIPETTE WITH GAUZE

ADJUST THE MENISCUS

DRAIN INTO TUBE/BEAKER

Question 13

(6) PARTS OF AUTOMATIC PIPETTE

Answer 13

PLUNGER

TIP EJECTOR

HANDLE

VOLUMETER

SHAFT

TIP

Question 14

PARTS OF AIR DISPLACEMENT PIPETTE

Answer 14

PISTON

DISPOSABLE TIP

Question 15

PARTS OF POSITIVE DISPLACEMENT PIPETTE

Answer 15

SHAFT

DISPOSABLE PISTON

DISPOSABLE CAPILLARY

PISTON SEAL

Question 16

WHICH PIPETTE IS VOLUME CORRESPONDS TO THE SELECTED ASPIRATION VOLUME

Answer 16

AIR DISPLACEMT PIPETTE

Question 17

WHICH PIPETTE IS PLASTIC TIP CONTAINS INTEGRATED PISTON

Answer 17

POSITIVE DISPLACEMENT PIPETTE

Question 18

READING OF VOLUME IN AUTOMATIC PIPETTE:

P20=
P200=
P1000=

Answer 18

P20 = 2-20UL MICROPIPETTE

P200 = 20-200UL MICROPIPETTE

P1000 = 200-1000UL MICROPIPETTE

Question 19

2 COMMON TECHNIQUES IN USING AUTOMATIC PIPETTE

Answer 19

FORWARD PIPETTING

REVERSE PIPETTING

Question 20

STEPS USING AUTOMATIC PIPETTE

Answer 20

SET VOLUME

ATTACH TIP

PRESS HOLD PLUNGER AT FIRST STOP

PLACE TIP IN LIQUID

SLOWLY RELEASE THE PLUNGER

RELEASE LIQUID INTO TUBE AT SECOND STOP

EJECT TIP INTO WASTE CONTAINER

Question 21

(9) PARTS OF SYRINGE

Answer 21

THUMB REST

PLUNGER

FINGER FLANGE

BARREL

RUBBER STOPPER

NEEDLE HUB

LUER LOCK

NEEDLE

BEVEL

Question 22

(8) PARTS OF ANALYTICAL BALANCE

Answer 22

DRAFT SHEILD

LEVEL INDICATOR

DISPLAY

LEVEL ADJUSTMENT FEET

POWER BUTTON

TARE BUTTON

BALANCE PAN

DOOR HANDLE

Question 23

(3) CALIBRATION TYPES OF THERMOMETER

Answer 23

TOTAL IMMERSION THERMOMETER (MERCURY COLUMN)

PARTIAL IMMERSION THERMOMETER (SPECIFIC DEPTH)

COMPLETE IMMERSION THERMOMETER (ENTIRE)