antibodies

Question 1

types of Ig

• present in secretions;
• tears, saliva

Answer 1

IgA

Question 2

which Ig rects to the following temperature

• (cold) reacts at 40 C to room temp:
• (warm) reacts at 37 C

Answer 2

• IgM
• IgG

Question 3

for any Ig molecule, only 1 type of (?) is present

Answer 3

light chain

Question 4

antibodies produced after the introduction of the antigen from the same species

Answer 4

isoantibodies/alloantibodies

Question 5

types of Ig

• serum half life: 6 days
• complement fixation: yes (best)
• crosses placenta: no

Answer 5

IgM

Question 6

bonds responsible to hold polypeptide chains

Answer 6

disulfide bonds

Question 7

structure of Ab

all immunoglobulin milecules are made up of (?)-chain polypeptide units that consists of (?) large chains called (?), and (?) smaller chains called (?)

Answer 7

• four
• 2
• heavy chains
• 2
• light chains

Question 8

reaction with antigen

responsible for immobilization of motile organisms and for cell clumping

Answer 8

agglutinins

Question 9

amino terminal or carboxyl terminal

• variable region
• responsible for antigen binding

Answer 9

amino terminal

Question 10

types if Ig

• serum half life: 23 days
• complement fixation: yes
• crosses placenta: yes

Answer 10

IgG

Question 11

this is where the BENCE JONES PROTEINS are discovered

Answer 11

light chains

Question 12

structure of Ab

elucidated in the 1950s and 1960, by:

Answer 12

gerald edelman & rodney porter

Question 13

reaction with antigen

can cause agglutination of RBC

Answer 13

hemagglutins

Question 14

isotype, allotype, or idiotype

heavy chains are unique to its immunoglobulin class

Answer 14

isotype

Question 15

types of Ig

• functions in immunoregulation
• heat-labile; “anti-idiotypic antibody”
• 2nd to appear on B cell surface
• role on B cell maturation

Answer 15

IgD

Question 16

isotype, allotype, or idiotype

genetic variation on the constant region

Answer 16

allotype

Question 17

types of Ig

• serum half life: 5 days
• complement fixation: alternative pathway
• crosses placenta: no

Answer 17

IgA

Question 18

INTERCHAN or INTRACHAN BONDS

• (normal) heavy chain to heavy chain/heavy to light chain (H-H/H-L): (?)
• (abnormal) light chain to light chain (L-L): (?)

Answer 18

• INTERCHAN BONDS
• INTRACHAN BONDS

Question 19

• serves as a linkage points for disulfide bonds between 2 monomers
• present on IgM and IgA

Answer 19

joining chain

Question 20

amino terminal or carboxyl terminal

• constant region
• determine the serological & physical chain of an antibody

Answer 20

carboxyl terminal

Question 21

heavy chains or light chains

• 23,0000 da
• has KAPPA & LAMBDA

Answer 21

light chains

Question 22

serum IgA or secretory IgA

• dimer; “antiseptic paint”:
• monomer; can’t fix complement:

Answer 22

• seretory IgA
• serum IgA

Question 23

heavy chains or light chains

• determine Ig class (IgA, IgD, IgE, IgG, IgM)
• 50,000 to 90,000 da
• consists of 440 amino acid residue

Answer 23

heavy chains

Question 24

the folded domains of the H chains line up with L chains to produce a cylindrical structure called (?)

Answer 24

immunoglobulin fold / barrel

Question 25

# subclass if IgG - cannot cross the placenta: - can cross the placenta: - cannot fix complement: - can fix complement:

Answer 25

- IgG2 - IgG1 - IgG4 - IgG3

Question 26

# types of Ig - least abundant - "reagenic antibody" - "nuisance antibody" - heat labile

Answer 26

IgE

Question 27

major antibody in the secondary immune response

Answer 27

IgG (IgM is primary)

Question 28

antigen is captured within the barrel by binding to a small number of amino acids at strategic locations on each chain known as (?)

Answer 28

hypervariable region

Question 29

# number of structure unit - composed of one structure unit: - composed of more than a single monomeric unit - composed of two structure unit: - composed of five structure unit:

Answer 29

- momomer - polymer - dimer - pentamer

Question 30

- substances produced in response to antigenic stimulation that are capable of specific interaction with provoking immunogen - serum factors in the blood formed in response to foreign substance exposure - can be visualized by electrophoresis

Answer 30

antibodies/immunoglobulins

Question 31

# types of Ig - pentameric form - aka "macroglobulin" - "starlike" (free) - "crab-like" (combined) - primary immune response

Answer 31

IgM

Question 32

# occurence of Ig - (natural) appear without any apparent stimulus: - (immune) appear following the introduction of an antigen:

Answer 32

- IgM - IgG

Question 33

primary agents for fragmentation

Answer 33

- PAPAIN - PEPSIN

Question 34

- allows flexibility - absent in IgM and IgE

Answer 34

hinge region

Question 35

antibodies produced after the introduction of the antigen from another species

Answer 35

heterophile antobodies

Question 36

# reaction with antigen act upon bacterial invaders, weakening their resistance to the phagocytizing action of leukocyte

Answer 36

opsonins

Question 37

# types of Ig - serum half life: 1-3 days - complement fixation: no - crosses placenta: no

Answer 37

IgD

Question 38

# theories he formulated the side-chain theory

Answer 38

paul ehrlich

Question 39

# theories he put forth a second major theory in the early 1900, the template theory

Answer 39

felix haurowitz

Question 40

# reaction with antigen agglutinins that are modified by heat in such manner that they can no longer bring out agglutination but still are capable of combining with specific agglutinogens

Answer 40

agglutinoids

Question 41

# gerald edelman, rodney porter, alfred nisonoff utilized PAPAIN

Answer 41

rodney porter

Question 42

- KAPPA is encoded on: - LAMBDA is encoded on:

Answer 42

- chromosome 2 - chromosome 22

Question 43

# theories who made the clonal selection theory (most acceptable theory)

Answer 43

niels jerne & macfarlane burnet

Question 44

# gerald edelman, rodney porter, alfred nisonoff utilized PEPSIN

Answer 44

alfred nisonoff

Question 45

# types of Ig - serum half life: 2-3 days - complement fixation: no - crosses placenta: no

Answer 45

IgE

Question 46

# gerald edelman, rodney porter, alfred nisonoff ultracentrifuge

Answer 46

gerald edelman

Question 47

# isotype, allotype, or idiotype genetic variation on the variable region

Answer 47

idiotype