AS MicroScopes

Question 1

What is the first lens light focused through in optical Microscope?

Answer 1

1. Light focused through condenser lens

Question 2

What is the first step in Optical MicroScope?

Answer 2

Light is focused through condenser lens then through
Specimen where certain wavelength are filtered.

Question 3

After light is passed through condenser lens in optical microscope where is light then passed?

Answer 3

Light then passes through objective lens then to eyepiece lens to produce a visible image.

Question 4

Which microscope has the highest Magnification?
Put the microscopes in order in descending order

Answer 4

Highest Magnification: TEM (Transmission Electron microscope)
2.SEM (Scanning Electron microscope)
3.Optical Microscope

Question 5

Which microscope has the highest resolution?
Put them in order in descending order

Answer 5

1.TEM and SEM
Then optical microscope.

Question 6

How does TEM electrons work?

Answer 6

Electrons pass through specimen and denser parts absorb more electrons so appear darker

Question 7

How does Scanning Electron Microscope?

Answer 7

Electrons bounce off specimen and are detected at multiple detectors.

Question 8

What is the first step using Eyepiece Graticule and calibrated stage micrometer to measure Actual Size?

Answer 8

Line up Eyepiece Graticule with Calibrated Stage Micrometer.

Question 9

What is the second step using Eyepiece Graticule and calibrated stage micrometer to measure Actual Size?

Answer 9

Count the number graticule division that correspond with set measurement on calibrated stage micrometer.

Question 10

What is the last step using Eyepiece Graticule and calibrated stage micrometer to measure Actual Size?

Answer 10

Calculate the distance in micrometer of one division on eyepiece graticule

Question 11

What are the limitations of using an electron Microscope?

Answer 11

1.Can’t view live specimen due to interior being a vacuum.

2.Specimen sliced very thinly

3.Require extensive staining process which leads to artefacts.

Question 12

What is the first step in Cell Fractionation?

Answer 12

Sample is homogenised and place in test tube with homogenate and centrifuge it and spin it at low speed

Question 13

Low speed centrifuge isolate what organelle?

Answer 13

Isolates nuclei and nuclei pellet is separated.

Question 14

After the first organelle is removed from homogenate what next is a pellet and removed?

Answer 14

At a higher speed the second thing organelle that is removed is mitochondria and chloroplasts.
Pellet at the bottom and removed

Question 15

What needs to be removed before you get a cell free liquid?

Answer 15

Higher speed centrifuge Endoplasmic Reticulum and Golgi (Body) form pellet and removed.

Question 16

what is the order of the organelles removed in cell fractionation?

Answer 16

Nucleus

Mitochondria and chloroplasts

Endoplasmic Reticulum and Golgi Body

Cell free Liquid.

Question 17

Which microscope produces a 2D image and which microscope produces a 3D image?

Answer 17

2D image is Transmission Electron Microscope (TEM)

3D image is Scanning Electron Microscope (SEM