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MTAP 1 FINALS > AUBF Chemical Urine Examination > Flashcards

AUBF Chemical Urine Examination Flashcards

(51 cards)

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1
Q

Benedict’s test does not detect

A

sucrose

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2
Q

Principle of benedict’s test

A

copper reduction

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3
Q

Reporting of Benedict’s test: No change in color (Blue solution)

A

Negative

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4
Q

Reporting of Benedict’s test: Green opacity, no precipitate

A

trace (+/-)

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5
Q

Reporting of Benedict’s test: Green solution with yellow precipitate

A

Positive (+)

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6
Q

Reporting of Benedict’s test: Green to yellow solution with yellow precipitate

A

Positive (++)

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7
Q

Reporting of Benedict’s test: Muddy orange solution with yellow precipitate

A

Positive (+++)

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8
Q

Reporting of Benedict’s test: Orange to brick red precipitate

A

Positive (++++)

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9
Q

Benedict’s test reaction result:

Cupric Ions (Blue Solution) + Reducing Sugar → (heat alkali) →

A

Carboxylic Acid + Cu2O (Cuprous Oxide) (Brick-Red precipitate)

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10
Q

SULFOSALICYLIC ACID PRECIPITATION (SSA) TEST Principle

A

Precipitation of urine protein by strong acid

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11
Q

Classic test for differentiation of urobilinogen and porphobilinogen

A

Watson-Schwartz Test

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12
Q

Butanol location in Watson-Schwartz Test

A

upper

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13
Q

Chloroform location in Watson-Schwartz Test

A

Lower

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14
Q

Turbidity grade and protein range:

No increase in turbidity

A

Negative: <6

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15
Q

Turbidity grade and protein range: Noticeable turbidity

A

Trace: 6-30

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16
Q

Turbidity grade and protein range: Distinct turbidity, no granulation

A

1+ : 30-100

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17
Q

Turbidity grade and protein range: Turbidity, granulation, no flocculation

A

2+: 100-200

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18
Q

Turbidity grade and protein range: turbidity, granulation, flocculation

A

3+: 200-400

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19
Q

Turbidity grade and protein range: clumps of protein

A

4+: >400

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20
Q

Analyte: Urobilinogen

Chloroform and Butanol result

A

Chloroform and Butanol: soluble

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21
Q

Analyte: Porphobilinogen

Chloroform and Butanol result:

Study These Flashcards
A

C and B: Insoluble

22
Q

Analyte: other ehrlich reacting compounds

Chloroform and Butanol result:

Study These Flashcards
A

C: Insoluble
B: Soluble

23
Q

Obsolete urine chemical test and analyte end result:

Heat and Acetic acid
Nitric Acid Test for albumin/protein

24
Q

Obsolete urine chemical test and analyte end result:

Benedict Test for Glucose (other reducing sugar)

Study These Flashcards
A

Brick Red Precipitate

25
Obsolete urine chemical test and analyte end result: Heat Precipitation Test (Bence Jones Protein)
Turbidity
26
Obsolete urine chemical test and analyte end result: Rothera for ketones
Red purple color
27
Obsolete urine chemical test and analyte end result: Gunning for Ketones
Iodoform Crystals (microscopic)
28
Obsolete urine chemical test and analyte end result: Gerhadt for Ketones
Bordeaux Red Color
29
Obsolete urine chemical test and analyte end result: Seliwanoff for Levulose
Orange to Red Color
30
Obsolete urine chemical test and analyte end result: Rubner for Lactose
Brick Red Precipitate
31
Obsolete urine chemical test and analyte end result: Gmelin for Bile
Play of Colors (Yellow, Red, Green)
32
Obsolete urine chemical test and analyte end result: Smith for Bile
Green Ring
33
Obsolete urine chemical test and analyte end result: Erlich for Urobilinogen
Cherry Red
34
Obsolete urine chemical test and analyte end result: Schlesinger for Urobilin
Fluorescent Green
35
Obsolete urine chemical test and analyte end result: Obermayer for Indican
Indigo blue to Indigo red
36
Obsolete urine chemical test and analyte end result: Gualac for Hemoglobin
Blue color (junction)
37
Obsolete urine chemical test and analyte end result: Ammonium sulfate for Hemoglobin
Precipitation of HGB
38
Obsolete urine chemical test and analyte end result: Thormalen for melanin
Prussian blue color
39
Obsolete urine chemical test and analyte end result: Sulkowitch for calcium
Turbidity
40
Obsolete urine chemical test and analyte end result: Fantus for chloride
white precipitate
41
Micral test normal protein range:
<10mg/dL or 100 mg/24 hours
42
Microalbuminuria range for micral test:
20-200 mg/L
43
Albuminuria range for micral test:
>200 mg/L
44
Indication of microalbuminuria and albuminuria
Kidney problems
45
Principle of Microalbumin Testing (Immunologic Testing)
Enzyme Immunoassay
46
Sensitivity of Micral test
0-10 mg/dL
47
Reagents for Microalbumin testing
Gold-labeled antibody B-Galactosidase Chlorophenol red galactoside
48
Interference for Micral Test
False-negative: Dilute urine
49
Immunodip principle
Immunochromographics
50
Sensitivity for Immunodip
1.2 to 8.0 mg/dL
51
Reagents for immunodip
Antibody-coated blue latex particles

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