Bacteria

Question 1

gram staining process acronyms

Answer 1

Fans Can Intimidate Dad Sadly

Question 2

outline gram staining process

Answer 2

1. fixation 2. add crystal violet 3. Add iodine 4. Decolourise with alcohol until no more purple comes off the side 5. Add safranin (pink)

Question 3

gram positive results

Answer 3

will stain purple due to sufficient peptidoglycan layer

Question 4

gram negative results

Answer 4

will stain pink- insufficient peptidoglycan layer to retain safranin

Question 5

Gram positive cocci’s

Answer 5

staplyloccocus

streptococcus

Question 7

which test is used to differentiate straphyloccocus

Answer 7

coagulase

Question 8

staphyloccus bacteria with a negative coagulase test

Answer 8

staphylococcus epidermidis

Question 9

staphyloccus bacteria with a positive coagulase test

Answer 9

Staphylococcus aureus

Question 10

which test is used to differnetiate streptooccus bacteria

Answer 10

Hemolysis

Question 11

partial hemolysis

Answer 11

alpha

Question 12

complete haemolysis

Answer 12

beta

Question 13

no haemoloysis

Answer 13

Y- gamma

Question 14

alpha streptococci

Answer 14

partial haemolysis

• Streptococcus pneumoniae

Question 15

Beta streptococci

Answer 15

complete haemolysis

• streptococcus pyrogenes

Question 17

gamma streptococci

Answer 17

no haemolysis

• Enterococci

Question 18

streptococcus or staphyloccocus

Answer 18

Gram positive streptococcus

Question 19

streptococcus or staphylococcus

Answer 19

gram positive staphylococcus

Question 20

name 4 gram positive bacillus

Answer 20

clostridium

listeria

cornyebacterium

lactobacillus

Question 21

name the gram negative cocci

Answer 21

nesseria meningitidis- diplococcus

Question 22

name all gram negative bacillus

Answer 22

Legionella

Yersinia

Escherichia

Vibrio (comma shaped)

Salmonella

Helicobacter (comma shaped)

Shigella

Enterobacter

Question 23

are gram negative bacteria usually cocci or bacillus

Answer 23

baciilus

Question 24

are gram posiitve bacteria usually bacillus or rod

Answer 24

mixed

Question 25

name me 3 encapsulated abcteria

–> which cause problems especially in patients with reduced spleen function

Answer 25

Neisseria meningitis

Haemophilus influenza

Streptococcus pneumoniae