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Flashcards in Bacterial Growth and Nutrition Deck (27):
1

anabolism

- construct molecules from smaller units
- primarily reductive

2

catabolism

- breaks down molecules into smaller units that are either oxidized to release energy, or used in other anabolic reactions
- primarily oxidative

3

Biomass

- What bacterium is made of

4

What bacterium is made of

- 55% protein
- 24% DNA/RNA
- 9% Lipid
- 6% Carb
- 3% other organics
- 1% inorganics

5

Macroelements

- Carbon
- Hydrogen
- Nitrogen
- Oxygen
- Phosphorus
- Sulfur
- Iron

6

Autotrophs

- get energy from the sun
- reduced inorganic chemicals

7

Heterotrophs

- steal energy in the form of reduced carbon

8

Chemotrophs

- chemicals as energy sources

9

Chemoautotrophs

- derive energy from chemical reactions
- synthesize all necessary compounds from CO2
- use inorganic energy sources

10

Chemoheterotrophs

- unable to fix carbon to form their own carbon compounds
- must ingest preformed organic molecules
- still obtain energy from oxidation of inorganic molecules

11

Phototrophs

- light as an energy source

12

photoheterotrophs

- organic electron donors

13

photoautotrophs

- inorganic electron donors

14

Reduced molecules have

- more energy
- more electrons

15

E. Coli's carbon/energy source

1. carbohydrate
2. amino acids
3. lipid
4 nucleotides

16

Carbon

- heterotrophs use organics to supply carbon
- autotrophs can "fix" CO2 from air

17

Hydrogen

- carbon source typically supplies H (H2O in autotrophs)

18

Oxygen

- Carbon source also typically supples O

19

Nitrogen

- Amino acids, NH4+, NO3-, (also N2-fixation)

20

Phosphorus

- almost entirely from inorganic P (PO42-)

21

Sulfur

- S-amino acids (cysteine), sulfate (SO42-)

22

Micronutrients

- typically inorganic (transition metals)
- typically required in microgram quantities
- often present in laboratory water and glassware
- serve structural/catalytic roles in specific enzymes

23

Manganese

- involved in phosphate transfer

24

Molybdenum

- involved in nitrogen transformations

25

Bug "Growth Factors"

- typically small organic compounds
- amino acids (protein synthesis)
- purines/pyrimidines (nucleic acid synthesis)
- vitamins (enzyme cofactors)
- others include heme, cholesterol, polyamides
- required in varying concentrations

26

defined media

- exact chemical composition is known

27

complex media

- chemical composition is poorly defined