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Flashcards in Translation Deck (13):
1

tRNA loading

- load with AA by tRNA synthetase
- attach covalently

2

ribosome

- 30S controls mRNA decoding site
- 50S contains peptidyl transferase that helps form peptide bonds

3

IF1

induces ribosome to dissociate

4

IF3

binds to 30S submit forming IF3-30S-mRNA complex to prevent reassociation of ribosome

5

IF2

- complexes with GTP and initiator tRNA-fmet
- directs binding of tRNA to 30S

6

IF3 dissociates

- 50S subunit reassociates
- GTP hydrolyzes - GDP, IF3, and Pi leave
- fmet-tRNA on top of start codon in P site

7

elongation

A site accepts next tRNA

8

Ef-Tu

- brings next tRNA with use of GTP and moves to A site
- forms peptide bond

9

23 subunit

- helps form peptide bond

10

Ef-G

- helps transfer tRNA from A site to P site with help of GTP
- tRNA goes to E site and leaves

11

Termination

- termination codon enters A site
- Ef-Tu can't bind - translation stalls

12

RF1/RF2

release polypeptide from tRNA in P site

13

RF3

removes RF1/RF2