Regulation DNA initiation Flashcards Preview

MB 414 > Regulation DNA initiation > Flashcards

Flashcards in Regulation DNA initiation Deck (8):
1

Regulating what

DnaA to # of A boxes

2

What happens at origin

each chromosome has 1 origin of rep located near DnaA gene

3

in fast growing cells

- origin gets replicated so now there are two

4

newly synthesized DNA

- hemi-methylated on old (parent strand)
- contains A box

5

SeqA

- binds to hemi-methylated strand and sequesters to cell membrane.
- DnaA has no access now

6

dam methylase

will methylate the new strand of the newly synthesized DNA if not sequestered to SeqA

7

DnaA

will bind to the fully synthesized strand

8

what does that make sure of

- to make sure that the origins fire at the same time once per cell cycle.
- prevents buildup of DnaA until GATC sequences fully methylated