Basics of microbio Flashcards Preview

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Flashcards in Basics of microbio Deck (18):
1

branching filamentous bacteria; gram stain

gram positive branching filamentous bacteria: actinomyces and nocardia (weakly acid fast)

2

bacteria w/o cell wall

mycoplasma. do not gram stain. contain sterols instead

3

what is special about mycobacteria

contain mycolic acid and have high lipid content. gram positive acid fast bacteria.

4

What is a gram variable bacteria?

gardnerella. rod shaped.

5

What are some bugs with gram stain limitations

treponema (syphilis- too thin to be visualized), mycobacteria, mycoplasma, legionella pneumophilia (mostly intracellular), rickettsia (intracellular), and chlamydia (intracellular and lacking muramic acid)
test legionella with a silver stain; test treponemes with dark field microscopy and fluorescent antibody staining

6

What bugs must bee sen with Giemsa staining?

chlamydia, borrelia, rickettsiae, trypanosomes, plasmodium

7

PAS stain

stains glycogen, mucopolysaccharides. used to diagnose Whipple disease (tropheryma whippleri)

8

Ziehl-Neelsen/carbol fuchsin stain

stains acid-fast organisms like nocardia and mycobacterium

9

india ink stain

cryptococcus neoformans

10

silver stain

fungi, legionella, helicobacter pyloir

11

chocolate agar with factor V (NAD+) and X (mehatin)

H. infuenzae

12

culture requirements for neisseria

VPN media (aka Thayer-Martin): vancomycin, polymyxin (inhbits other gram negatives), and nystatin (inhibits fungi)

13

culture requirements for B. pertussis

Bordet-Gengou agar

14

Culture requirements for C. diphtheriae

tellurite agar, loffler medium

15

Lowenstein-jenson agare

required for M. TB

16

Eaton agar with cholesterol

M. pneumo culture requirementes

17

culture requirements for legionella

charcoal yeast extract agar with cysteine and iron

18

Culture for fungi

sabouraud agar