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BIOCHEM > Bile > Flashcards

Bile Flashcards

1
Q

Physical characteristic of bile

A

clear, golden yellow, slightly viscid fluid

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2
Q

Bile is produced by what?

A

Hepatocytes

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3
Q

Where is bile stored in?

A

Gallbladder

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4
Q

Bile functions for?

A

Digestion and Emulsification of fat

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5
Q

What are the components of bile?

A

Bile acids/salts, Phospholipids, Cholesterol, Bilirubin

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6
Q

Name the three Bile acids/salts

A

Cholic, Chenodeoxycholic, Deoxycholic

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7
Q

How much is the composition of Organic matter in bile?

A

3/4

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8
Q

How much is the composition of Inorganic matter in bile?

A

1/4

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9
Q

Name the Organic matters of Bile
(Clue: BA, BP, C, P, M, U, A)

A

Bile acids, Bile pigments, Cholesterol, Phospholipid (Lecithin), Mucin, Urea, ALP

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10
Q

Percentage of Cholic acids

A

26-60%

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11
Q

Percentage of Chenodeoxycholic acids

A

30-50%

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12
Q

Percentage of Deoxycholic acid

A

5-25%

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13
Q

Name the Inorganic matters in bile
(Clue: C, S, P)

A

Chloride (Cl), Sodium Bicarbonate (HCO3), Potassium (K)

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14
Q

Volume of bile in ml/24hrs

A

500-700 ml/24hrs

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15
Q

pH level of bile

A

7-8.5

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16
Q

The increased fat or lipids in the stool where the body is unable to absorb fats (sign of malabsorption)

A

Steatorrhea

17
Q

Reagent used in test for Bile acids

A
  • Conc. Sulfuric Acid
  • 5% sucrose solution
18
Q

Reagent used for test for Bile pigments

A
  • Filter paper
  • conc. Nitric acid
19
Q

Reagents used in test for Bilirubin

A
  • 2.5% of Barium chloride
20
Q

Reagents use for test for Cholesterol procedure A

A
  • Chloroform
  • Conc. Sulfuric
    acid
21
Q

Reagents used for test for Cholesterol procedure B

A
  • Chloroform
  • acetic anhydride
  • Conc. Sulfuric
    acid
22
Q

What is the name of the test for Bile Acids?

A

Pettenkofer’s test

23
Q

What is the name of the test for Bile Pigments?

A

Rosenbach’s or Gmelin’s test

24
Q

What is the name of the test for Bilirubin?

A

Fouchet’s test

25
Q

What is the name of the test for Cholesterol (Procedure A)?

A

Salkowski

26
Q

What is the name of the test for Cholesterol (Procedure B)?

A

Lieberman-Burchard

27
Q

Expected result for Bile acids

A

Red to bluish violet upon standing

28
Q

Expected result for Bile pigments

A

Yellow spot with rings of red, violet, blue and green

29
Q

Expected result for Bilirubin

A

Green color

30
Q

Expected result for Cholesterol procedure A

A

Red color

31
Q

Expected result for Cholesterol procedure B

A

Green color

32
Q

What are the two primary bile acids?

A

Cholic and Chenodeoxycholic Acid

33
Q

What are the two secondary bile acids?

A

Deoxycholic and Lithocholic acid

BIOCHEM (10 decks)

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